Ecoli Catalog 2014

 

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Ecoli Catalog 2014 - PCR Diagnostics Kits

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CATALOG PCR Diagnostics Kits Molecular Biology Reagents Human SNP Kits Forensic and Paternity STR Kits

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Content of Catalog Legend Explanation of Symbols in Tables ........................................................................................ 7 About Us.................................................................................................................................. 8 Index of Catalog ...................................................................................................................... 9 Ordering ................................................................................................................................ 10 PCR Diagnostics Kits 1. Sexually Transmitted Infections Chlamydia trachomatis ............................................................................................... 11 Neisseria gonorhoeae ................................................................................................ 11 Treponema pallidum .................................................................................................. 12 Trichomonas vaginalis................................................................................................ 12 Mycoplasma genitalium .............................................................................................. 12 Mycoplasma hominis .................................................................................................. 12 Ureaplasma species ................................................................................................... 13 Ureaplasma species differentiation .............................................................................. 13 Gardnerella vaginalis ................................................................................................. 13 Candida albicans ....................................................................................................... 13 MultiPlex PCR Detection Kits ....................................................................................... 14 N. gonorrhoeae / T. vaginalis .............................................................................. 14 C. trachomatis / Ureaplasma / M. genitalium / M. hominis...................................... 14 C. albicans / C. glabrata / C. krusei ...................................................................... 14 C. trachomatis / Ureaplasma /M. genitalium.......................................................... 14 C. trachomatis / Ureaplasma ............................................................................... 14 M. hominis / G. vaginalis ..................................................................................... 14 T. vaginalis / N. gonorrhoeae / C. trachomatis ...................................................... 14 N. gonorrhoeae /C. trachomatis / M. genitalium / T. vaginalis ................................ 14 G. vaginalis / Lactobacillus species ....................................................................... 14 C. trachomatis / Ureaplasma / M. hominis ........................................................... 14 Floroscenosis - bacterial vaginosis ........................................................................ 15 Floroscenosis / Candida ............................................................................................. 15 Floroscenosis / Mycoplasma ....................................................................................... 15 Floroscenosis / Aerobes ............................................................................................. 15 2. Human Papilloma virus Infections High-Risk Human Papilloma virus Infections ................................................................ 16 Low-Risk Human Papilloma virus Infections ................................................................ 16 3. TORCH Infections Toxoplasma gondii .................................................................................................... 17 Parvovirus В19 .......................................................................................................... 17 Rubella virus ............................................................................................................. 18 4. Herpes-virus Infections Cytomegalovirus........................................................................................................ 19 Epstein-Barr virus ...................................................................................................... 19 Varicella Zoster virus ................................................................................................. 19 Human Herpes virus 6 ............................................................................................... 20 Herpes Simplex virus HSV-1, HSV-2 ............................................................................ 20 Herpes Simplex virus Genotyping ................................................................................ 21 3

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Epstein-Barr virus / Cytomegalovirus / Human Herpes virus 6 ................................. 21 Herpes Simplex virus / Cytomegalovirus................................................................ 21 5. Purulent Septic Infections MRSA ....................................................................................................................... 22 Streptococcus agalactiae ............................................................................................ 22 Pseudomonas aerigunosa ........................................................................................... 22 Streptococcus pyogenes ............................................................................................. 22 Genetic Markers of Antibiotic Resistance ...................................................................... 22 6. Respiratory Infections Avian Influenza (Bird Flu), Subtype H5N1 .................................................................... 23 Influenza virus А/H1 (Swine Flu) ................................................................................. 23 Influenza virus А/В .................................................................................................... 24 Adenovirus ................................................................................................................ 24 Parainfluenza virus..................................................................................................... 24 Mycobacterium tuberculosis complex ........................................................................... 24 Respiratory-Syncytial virus (hRSV) .............................................................................. 25 Legionella pneumophila .............................................................................................. 25 MERS and SARS – Coronavirus ................................................................................... 25 MultiPlex PCR Detection Kits ....................................................................................... 26 Acute Respiratory Viral Infections (ARVI) ............................................................. 26 Bordetella multi .................................................................................................. 26 Mycoplasma pneumoniae / Chlamydophila pneumoniae ......................................... 26 7. Neuro Infections Enterovirus................................................................................................................ 27 Poliovirus .................................................................................................................. 27 Listeria monocytogenes .............................................................................................. 27 MultiPlex PCR Detection Kits ....................................................................................... 27 Neisseria meningitidis / Haemophilus influenzae / Streptococcus pneumoniae .......... 27 8. Intestinal Infections Campylobacter species ............................................................................................... 28 Helicobacter pylori ..................................................................................................... 28 Clostridium difficile..................................................................................................... 28 Salmonella typhi ........................................................................................................ 28 Food Pathogen Detection Kits Cronobacter sakazakii.......................................................................................... 29 Shigella spp. and EIEC ........................................................................................ 29 EHEC ................................................................................................................. 29 Salmonella spp. ................................................................................................. 29 MultiPlex PCR Detection Kits ....................................................................................... 30 Rotavirus / Norovirus /Astrovirus .......................................................................... 30 All Screen (Shigella + EIEC / Salmonella / Campylobacter / Rotavirus / Norovirus / Astrovirus / Adenovirus) .................................................................... 30 Shigella and EIEC / Salmonella / Campylobacter .................................................... 30 Escherichioses ........................................................................................................... 30 MultiPlex PCR Detection Kits ....................................................................................... 21 4

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9. Especially Dangerous and Feral Herd Infections Vibrio cholerae .......................................................................................................... 31 Bacillus anthracis ....................................................................................................... 31 Brucella species ......................................................................................................... 31 Dengue fever virus .................................................................................................... 31 Leptospira species ..................................................................................................... 32 Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. garinii) ............ 32 Tick-borne encephalitis virus ...................................................................................... 32 West Nile fever virus.................................................................................................. 32 Crimean-Congo hemorrhagic fever virus ...................................................................... 33 Yersinia pestis ........................................................................................................... 33 Coxiella burnetii ........................................................................................................ 33 MultiPlex PCR Detection Kits ....................................................................................... 33 TBEV / B. burgdorferi sensu lato / A. phagocytophillum / E. chaffeensis / E. muris ... 33 10. Oncological Disease Leukosis Quantum M-bcr............................................................................................ 34 11. HIV and HIV-associated Infections HIV ......................................................................................................................... 35 Identification of Drug Resistant Mutations ................................................................... 36 Pneumocystis jirovecii ................................................................................................ 36 Cryptococcus neoformans .......................................................................................... 36 12. Hepatitis Viruses Infections Hepatitis A virus ........................................................................................................ 37 Hepatitis B virus ........................................................................................................ 37 Hepatitis C virus ........................................................................................................ 38 Hepatitis D virus ........................................................................................................ 39 Hepatitis G virus ........................................................................................................ 39 MultiPlex PCR Detection Kits ....................................................................................... 39 HCV/HBV/HIV..................................................................................................... 39 HBV/HDV ........................................................................................................... 39 Genoscreen IL28B .............................................................................................. 39 13. Additional Kits DNA and RNA Extraction Kits ...................................................................................... 40 Reverse Transcription Kits .......................................................................................... 41 Electrophoretic Detection ........................................................................................... 41 Transport and Storage Media ..................................................................................... 41 14. Human SNP Kits Description Principle............................................................................................................. 42 Methodology of Human SNP Kits .......................................................................... 43 Cardiovascular Diseases ............................................................................................. 44 Lipid Metabolism ....................................................................................................... 45 Pathology of Blood Coagulation System ....................................................................... 46 Breast/Ovarian Cancer ............................................................................................... 47 Osteoporosis ............................................................................................................. 48 5

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Diabetes mellitus ....................................................................................................... 48 Obesity ..................................................................................................................... 49 Crohn’s Disease ......................................................................................................... 50 Pharmacokinetics ....................................................................................................... 50 Immune System ........................................................................................................ 53 Sport Genetics ........................................................................................................... 54 PYRO-prep ................................................................................................................ 54 Forensic and Paternity STR Kits ............................................................................. 55 COrDIS Plus .............................................................................................................. 55 COrDIS-11 ................................................................................................................ 57 COrDIS-9 .................................................................................................................. 58 List of Analyzed STR Loci............................................................................................ 59 Ordering .......................................................................................................................... 60 6

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Legend Explanation of Symbols in Tables What Cycler Can Be Used? Cycler type abbreviation in catalog number determines, for what qPCR cycler is a detail manual included: • • • • • RG Rotor-Gene Q/3000/6000, iQ iCycler iQ/iQ5/CFX (Bio-Rad), Mx Mx3000P/3005P (Stratagene), SC SmartCycler (Cepheid), ...for non-listed Real-Time PCR cycler, ask us for application data. Kits in non-aliquoted format can be used on any cycler with needed channels: • • • • Rotor-Gene Q/3000/6000, iCycler iQ/iQ5/CFX (Bio-Rad), Mx3000P/3005P (Stratagene), SmartCycler (Cepheid), • • • • • Bioneer Exicycler™ 96, ABI™ 7300/7500/StepOne, EcoqPCR™ (Illumina), LineGeneK® (Bioer), ...and similar. Real-Time detection Catalog number Fluorescent End-Point detection TaqMan™ technology based kits. PCR is performed in a standard thermal cycler, analyzis is performed in a multichannel fluorescent reader ALA 1/4. Number of reactions including controls Shelf life (months)  R-X1-F(RG,iQ,Mx,SC)-CE  X1-100-R0,2-FEP-CE  X1-100-R0,2-CE  110 :: :: 100 100 ⑥ ⑨ ⑫ Electrophoresis detection Kit reagents are non-aliquoted Kits in non-aliquoted format can be used on any cycler with needed channels: • • • • • • • • • Kit reagents are aliquoted PCR reactions are aliquoted in individual PCR tubes, ready-to-use Rotor-Gene Q/3000/6000, iCycler iQ/iQ5/CFX (Bio-Rad), Mx3000P/3005P (Stratagene), SmartCycler (Cepheid), Bioneer Exicycler™ 96, ABI™ 7300/7500/StepOne, EcoqPCR™ (Illumina), LineGeneK® (Bioer), ...and similar. CE IVD CE-marked kits, comply with EU Directives 93/42/EEC and 98/79/EC (Medical Products and IVD) In vitro diagnostics 7

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About Us Ecoli Ltd. is an european company situated in Slovakia with worldwide distribution reach. (Central Research Institute for Epidemiology, Moscow) for clinical, veterinary diagnostics and human genome testing. Kits are designed according to laboratory facilities for electrophorese, FEP (Fluorescence End-Point) and Real-Time PCR detection, as well as for pyrosequencing technology. PCR diagnostic kits have very high sensitivity, high specificity and a very reasonable price. Most of the kits are produced as in vitro diagnostics and have CE certificate. MultiPlex Real-Time/FEP PCR kits allow to establish the presence of several infectious agents (multiplex analysis) during just one reaction. That increases the speed of detection and reduces the cost of examinations. New product line consists of wide range of SNP kits. Human SNP kits, by using of pyrosequencing technology, allow to determine and quantify specific point mutations in a human genome by very simple and nonexpensive way. Forensic and paternity STR multiplex kits open new possibilities for rapid and cost effective analysis of unknown DNA from standard and trace samples, as well as analysis of degraded DNA. Ecoli Ltd. provides more than 350 different types of AmpliSens® PCR diagnostics kits produced by CRIE Ecoli Distribution Network • • • • • • • • • • • • • • • • • Afghanistan Albania Algeria Bangladesh Bulgaria Chile China Croatia Czech Republic Ecuador Germany Philippines France Greece Georgia Ireland Jordan • • • • • • • • • • • • • • • • • • Kosovo Kuwait Macedonia Hungary Malta Morocco Peru Poland Portugal Romania UAE Serbia Spain Sweden Switzerland UK Uganda Venezuela Ecoli Headquarters 8

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PCR Diagnostics Kits Index of Catalog 1. Sexually Transmitted Infections................................................. 11 2. Human Papilloma Virus Infections ............................................ 16 3. TORCH Infections ...................................................................... 17 4. Herpes-virus Infections ............................................................. 19 5. Purulent Septic Infections ......................................................... 22 6. Respiratory Infections ............................................................... 23 7. Neuro Infections........................................................................ 27 8. Intestinal Infections ................................................................... 28 9. Especially Dangerous and Feral Herd Infections ....................... 31 10. Oncological Disease ................................................................ 34 11. HIV and HIV-associated Infections .......................................... 35 12. Hepatitis Viruses Infections ..................................................... 37 13. Additional Kits ......................................................................... 40 14. Human SNP Kits / Forensic and Paternity STR Kits .................. 42 9 1 2 3 4 5 6 7 8 9 10 11 12 13 14

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Ordering How to Order Ordering can be done by sending to: • Email: ecoli@ecoli.sk • Fax: +421 2 6478 9040 • Address: Ecoli s.r.o. Studenohorská 12 841 03 Bratislava Slovak Republic Shipping Shipping cost is calculated for every shipment separately, as every box has different dimensions and weight. This system is customer-friendly because you pay for real shipping costs. Terms of Payment Ecoli s.r.o. accepts payments by wire transfer. The shipment will be sent out to you app. within 4 weeks after deadline. If you do not receive confirmation of your order, please contact us as soon as possible. Be Informed About DEADLINES! Ask for regular sending of information about our deadlines for summary order to the manufacture. Sending of your order before deadline will reduce delivery time to the minimum (app. 4 weeks). If you send your order after deadline, it will be processed to the next term of deadline. Required Information Following information is required in an order: • Product names • Catalog numbers • Specification (like number of reactions) • Shipping address • Billing address • VAT number (EU only) • Contact person • Telephone or cell number Customer Care We are committed to provide supreme services for our customers. All inquiries are answered and to all technical questions is given high priority and our full attention. 10

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PCR Diagnostics Kits Sexually Transmitted Infections Sexually transmitted disease (STD), also known as a sexually transmitted infection (STI), or venereal disease (VD), is an illness that has a significant risk of transmission between humans by means of human sexual behavior. While in the past these illnesses have mostly been referred to as STDs or VD, in recent years the term sexually transmitted infections (STIs) has been preferred, as it has a broader range of meaning; a person may be infected, and may potentially infect others, without having a disease. Some STIs can also be transmitted via the use of IV drug needles after its use by an infected person, as well as through childbirth or breastfeeding. STI is a broader term than STD. An infection is a colonization by a parasitic species, which may not cause any adverse effects. In a disease the infection leads to impaired or abnormal function. In either case the condition may not exhibit signs or symptoms. Increased understanding of infections like HPV, which infects most sexually active individuals, but cause disease in only a few has led to increased use of the term STI. The diseases on this list are most commonly transmitted solely by sexual activity. Many infectious diseases, including the common cold, influenza, pneumonia and most others that are transmitted person-to-person can also be transmitted during sexual contact, if one person is infected. However, even though these diseases may be transmitted during sex, they are not considered STDs. MultiPlex Real-Time PCR technology allows to use primers and probes for several (for up to 5) DNA targets in one tube. Amplification products identification runs for each DNA target on a different optical channel. Sensitivity of these tests are not affected by changing the number of infections. Each mono- and multiplex PCR kit contains independent Internal Control (IC) for determination of DNA extraction efficiency and PCR process. Presence of the Internal Control signal/band shows, that DNA extraction process and amplification steps were sufficient for significant results interpretation. 1  Chlamydia trachomatis  Neisseria gonorrhoeae Chlamydia is a common STD caused by Chlamydia trachomatis, which can damage a woman's reproductive organs. Even though symptoms of chlamydia are usually mild or absent, serious complications can occure, like pelvic inflammatory disease or ireversible damage, including infertility. In men, the infection is usually symptomatic, with dysuria and a discharge from the penis. Untreated chlamydial infection in men can spread to the epididymis. Most women with chlamydial infection have minimal or no symptoms, but some develop. Chlamydial infection in newborns can cause ophthalmia neonatorum. AmpliSens® Ch. trachomatis PCR kits are built for fast and accurate detection or quantification of the pathogen in clinical samples - urogenital, rectal and throat swabs, urine, eye discharge and prostate secretion. Kits contain Internal Control for detection of DNA extraction efficiency, and amplification process. Analytical sensitivity is 5 x 102 copies/ml (qPCR). For detection, FAM/Green and JOE/Yellow channels are needed. Gonorrhea is a common STD caused by the bacterium N. gonorrhoeae. The usual symptoms in men are burning with urination and penile discharge. Women are asymptomatic half the time or have vaginal discharge and pelvic pain. Infection of the genitals in females can result in pelvic inflammatory disease if left untreated, which can result in infertility. In both men and women if gonorrhea is left untreated, it may spread locally causing epididymitis, disseminated infections can result in endocarditis, meningitis or gonococcal dermatitis-arthritis syndrome. Neonatal gonorrheal conjunctivitis can lead to corneal scarring or perforation, resulting in blindness in the neonate. AmpliSens® Neisseria gonorrhoeae Screen kit is a qualitative PCR kit and is recommended for screening of clinical samples. Due a new design, only one pair of primers + probe are used. Analytical sensitivity is 5 x 102 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed.       R-B1-F(RG,iQ)-CE R-B1(RG)-CE R-B1(iQ)-CE R-B1-100-FT(RG,iQ,Mx)-CE B1-100-R0,2-FEP-CE B1-100-R0,2-CE  :: :: QUANTITATIVE - RUO  :: :: RUO 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨  R-B51-F(RG,iQ)-CE  R-B56-F(RG,iQ)-CE NEW screen test screen test  B51-100-R0,2-FEP-CE  B56-100-R0,2-FEP-CE NEW   :: :: 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨ For DNA isolation use DNA-sorb-AM For DNA isolation use DNA-sorb-AM  - Real-Time;  - FEP;  - Elfo; :: - aliquoted form;  - non-aliquoted form (usable cyclers see page 7) 11

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PCR Diagnostics Kits Sexually Transmitted Infections 1  Treponema pallidum  Mycoplasma genitalium Infection by T. pallidum has diverse clinical manifestations initial genital tract lesion followed by disseminated lesions and cardiovascular and neurologic problems and CNS disease manifested as acute syphilitic meningitis. Infection during pregnancy results in numerous birth defects or fetal death. Infections in adults are usually chronic, death or serious disability is rare. AmpliSens® Treponema pallidum PCR kits are amplification tests for qualitative detection of T. pallidum DNA in the clinical materials (scrapes/swabs of urogenital tract mucous membranes; serous exudate of vesicles, ulcers or erosions). Internal Control allows to control DNA extraction efficiency, as well as amplification process. Analytical sensitivity is 1 x 103 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed.  R-B20-F(RG,iQ)-CE  B20-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  :: 110 ⑨ 110 ⑨ Mycoplasma genitalium is an often asymptomatic, bacterial, STD which bears some similarities to gonorrhoea and chlamydia. Because M. genitalium often occurs in association with other infections in both men and women, it is quite difficult to diagnose the condition on its own. M. genitalium in women has been linked to conditions such as bacterial vaginosis, cervicitis, pelvic inflammatory disease and endometritis. M. genitalium has also been found in women who have given birth prematurely. Often, M. genitalium is diagnosed in men who suffer from urethritis (inflammation of the urethra) which is not caused by gonorrhoea or chlamydia. AmpliSens® M. genitalium PCR kits are built for detection of the pathogen in clinical materials (cervical, urethral scrapes/ swabs, urine sediment, prostate gland secrete). Kits contain Internal Control for detection of DNA extraction efficiency, as well as for control of amplification process. Analytical sensitivity is 1 x 103 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed.  Trichomonas vaginalis  R-B4-F(RG,iQ)-CE  R-B4(RG)-CE  R-B4-100-FT(RG,iQ,Mx)-CE QUANTITATIVE T. vaginalis is a parasitic protozoan flagellate, generally restricted to the genitourinary tract by the host's immune system and is the etiological agent of human trichomoniasis. In women symptoms of infection include vaginal secretion that is scanty and mixed with mucus; malodorous discharge that is frothy, yellow or green, mycopurulent and copious. Complications may result in cervical erosion, cervical cancer, infertility, adnexitis, pyosalpinx and endometritis. Premature rupture of the placental membranes can occur in pregnant women, resulting in premature birth and low-birth weight. In men is the prevalence lower and infection is often asymptomatic. Infection in men can be present in the prostate, seminal vesicles and epididymis. Complications are rare, but can potentially lead to genitourinary inflammation disease, sterility, scanty, clear to mucopurulent discharge, dysuria, non-gonococcal urethritis, prostatitis and urethral disease. AmpliSens® T. vaginalis PCR kits are qualitative amplification tests for fast and accurate detection of the pathogen in clinical material. Kits contain Internal Control that allows detection of DNA extraction efficiency as well as amplification process. Analytical sensitivity is 5 x 102 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed.  B4-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  ::  :: 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨  Mycoplasma hominis Mycoplasma species are the smallest free-living organisms without cell wall, capable of self-replication. M. hominis exists in parasitic and saprophytic state. There is evidence, that M. hominis may be implicated in pelvic inflammatory disease, which may cause ectopic pregnancy. This bacterium prospers in the environment created by other G- bacteria implicated in bacterial vaginosis and may be a cause of preterm delivery or miscarriage. It may also be implicated in postpartum fever, because it may be a cause of endometritis. M. hominis is also suspected to be the cause of neonatal infections, including conjunctivis, respiratory distress, fever, meningitis, abscesses and congenital pneumonia, which occurs a few hours after birth. In adults, M. hominis may be implicated in pharyngitis, septicaemia, lung, as well as joint and wound infections. AmpliSens® Mycoplasma hominis PCR kits contain Internal Control for control of of DNA extraction and amplification processes. Analytical sensitivity is 1 x 10 3 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed.  R-B6-F(RG,iQ)-CE  B6-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  :: 110 ⑨ 110 ⑨  R-B3-F(RG,iQ)-CE  R-B3(RG)-CE  R-В3-100-FT(RG, iQ, Mx)-CE NEW QUANTITATIVE  B3-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  ::  :: 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨ 12  - Real-Time;  - FEP;  - Elfo; :: - aliquoted form;  - non-aliquoted form (usable cyclers see page 7)

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PCR Diagnostics Kits Sexually Transmitted Infections  Ureaplasma species  Gardnerella vaginalis Ureaplasma spp. causes bacterial infection, generally asymptomatic in nature, that is sexually transmitted. The bacteria can survive in the reproductive tract for many years undetected, until a patient is specifically tested for the infection. Infection is very similar to Mycoplasma, so it is recommended to test both bacteria in case of syndroms, described by Mycoplasma kits. AmpliSens® Ureaplasma spp. PCR kits are built for fast detection (without differentiation) of the pathogen in clinical material (cervical, urethral scrapes/swabs, urine sediment, prostate gland secrete). Kits contain Internal Control for detection of DNA extraction efficiency and control of amplification. Analytical sensitivity is 1 x 103 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed. Gardnerella vaginalis is just one of many causes of bacterial vaginosis caused by an increased production of the naturally occurring bacteria G. vaginalis. It is presumed to be a sexually transmitted disease and is often found in conjunction with a variety of other anaerobic bacteria. The most common symptom of G. vaginalis infection is a "fishy" smelling discharge and gray-white secretions. AmpliSens® G. vaginalis PCR kits are built for fast and accurate detection of the pathogen. PCR kits contain Internal Control for detection of DNA extraction efficiency, as well as control of amplification process. Analytical sensitivity is 1 x 104 copies/ml. For detection, FAM/Green and JOE/Yellow channels are needed. 1  R-B7-F(RG,iQ)-CE  B7-100-R0,2-FEP-CE  R-B2-F(RG,iQ)-CE  R-B2(RG)-CE  R-B2-100FT(RG,iQ,Mx)-CE  :: QUANTITATIVE 110 ⑨ 110 ⑨ 110 ⑨  :: 110 ⑨ 110 ⑨ For DNA isolation use DNA-sorb-AM  For DNA isolation use DNA-sorb-AM  Candida albicans  Ureaplasma spp. differentiation U. parvum/U. urealyticum PCR kits are in vitro nucleic acid amplification tests for qualitative detection and differentiation of U. parvum and U. urealyticum DNA in clinical materials (scrapes/swabs of urogenital tract mucous membranes; urine sediment; secret of the prostate gland). Kits contain Internal Control for detection of DNA extraction efficiency, as well as for control of amplification pro-cess. Analytical sensitivity is 1 x 103 copies/ml. In AmpliSens® Ureaplasma spp. differentiation PCR kits, U. parvum DNA is detected on the FAM/Green channel, U. urealyticum DNA is detected on JOE/Yellow/HEX channel and Internal Control is detected on the ROX/Orange channel. Candida sp. cause a wide spectrum of diseases, ranging from superficial mucocutaneous disease to invasive illnesses, such as hepatosplenic candidiasis, Candida peritonitis and systemic candidiasis. Local and systemic disease caused by Candida spp. has resulted in numerous new clinical syndromes, the expression of which depends primarily on the immune status of the host. Although Candida most frequently infects the skin and mucosal surfaces, it can cause systemic infections manifesting as pneumonia, septicaemia or endocarditis in severely immunocompromised patients. There does not appear to be significant difference in pathogenic potential of different Candida strains, therefore establishment of infection appears to be determined by host factors and not by the organism itself. However, the ability to assume various forms may be related to the pathogenicity of the organism. AmpliSens® Candida albicans PCR kits are qualitative tests and contain Internal Control which must be used in the isolation procedure in order to control the isolation process of each individual specimen and to identify possible PCR reaction inhibition. Analytical sensitivity is 1 x 103 copies/ml. For detection, 2 channels - FAM/Green and JOE/Yellow are needed.  R-B19-F(RG,iQ)-CE  R-B19(RG)-CE  R-B19-100-FT(RG,iQ,Mx)-CE  :: QUANTITATIVE 110 ⑨ 110 ⑨ 110 ⑨ 110 ⑨  B19-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  ::  R-F1-F(RG,iQ)-CE  F1-100-R0,2-FEP-CE For DNA isolation use DNA-sorb-AM  :: 110 ⑨ 110 ⑨  - Real-Time;  - FEP;  - Elfo; :: - aliquoted form;  - non-aliquoted form (usable cyclers see page 7) 13

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