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imagesourcetm user manual all possible care has been taken in the preparation of this publication but spectronics corporation accepts no liability for any inaccuracies that may be found spectronics corporation reserves the right to make changes without notice both to this publication and to the product that it describes no part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without the prior permission of the copyright owner published by spectronics corporation 2006

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contents imagesource initial setup 1 introduction and overview 1-1 connecting the system 1-2 software overview 1-3 getting started with imagesource 2 starting up 2-1 acquiring the image 2-2 saving the image 2-3 imagesource user manual contents

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introduction and overview introduction this manual gives full instructions for setting up imagesourcetm and provides basic operations of imagesource acquisition software the manual is divided into two parts initial setup and getting started with imagesource initial setup gives a brief overview of the imagesource hardware software and instructions for connection of necessary components getting started part of the manual takes you through basic procedures for capturing and saving an image overview imagesource is an affordable fully automated image acquisition system developed for use with laboratory grade 8-bit ccd camera connected to a pci prymo card imagesource data acquisition kit gl-3100 is supplied with pci prymo card image acquisition board ccd camera cables specific to imagesource acquisition board cd rom with licensed imagesource software and user manuals imagesource 2006.pdf this installation guide referenceimagesource.pdf detailed software operation note imagesource must be used with a pc that has windows 98se 2000 or xp operating system cpu must also have a spare pci slot for installation of prymo card imagesource user manual introduction 1-1

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imagesource connection connecting the system 1 turn on the pc customer supplied and load the imagesource analysis software into cd rom drive 2 the installation program will start automatically if it does not locate the cd rom drive in windows explorer and double-click the setup.exe follow instructions on screen and complete the software installation 3 turn off computer follow normal shut down procedures remove the cover and locate spare pci slot carefully remove the prymo card acquisition board from packaging and install card into slot appropriately 4 replace the cover of the computer and connect peripheral computer components monitor keyboard mouse etc 5 prepare the connection of the ccd camera gl-0100 or customer supplied to the card by attaching dual connector end to top of camera/hood assembly and the other to the acquisition board serial port on the computer 6 turn on the computer and restart the imagesource software message of successful installation will be indicated 7 imagesource is now ready for use with a transilluminator light source and ccd camera customer supplied imagesource user manual connection 1-2

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software overview imagesource acquisition software imagesource program is used to control the camera hardware to capture images and to process the captured images in particular imagesource software can control amount of light and exposure length while viewing a live image from the camera you can use imagesource to capture images from a live image as a single frame capture in a series with the same or variable exposures using auto exposure software capabilities the patented extended dynamic range edr feature allows you to stretch the dynamic range of the ccd camera in particular it increases the detail in low intensity areas of the image allowing you and analysis software like imageaidetm to distinguish features in dark parts of the object that would otherwise be undetectable without saturating the lighter areas the neutral field correction provided by imagesource allows you to correct for uneven illumination while maintaining good laboratory practice glp a histogram display allows you to maximize the image contrast without losing any detail for presentation purposes you can process captured images to create a negative image and increase or decrease the sharpness you can also crop images or flip them horizontally or vertically imagesource also allows you to add graphical and textual annotations then save as separate file from the image for further course of action all in accordance with glp all processing is performed on a copy of the original image which is saved with the original image original and processed images can be exported to files using a wide range of standard graphics formats and you can choose whether or not to engrave the annotations on the exported image imagesource user manual software overview 1-3

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getting started with imagesource this chapter gives a quick introduction to using your imagesource system taking you through the processes of starting up capturing and saving an image starting up 1 make sure the power leads from all components are connected to a live supply 2 place sample on the surface of the transilluminator uv or white light source toward the middle of the viewing field gently position the camera/hood assembly on top of the transilluminator 3 turn on the main power switches for transilluminator computer monitor and printer if applicable windows should default to imagesource if not double click shortcut icon on screen to imagesource software 4 when imagesource starts up the first time you will be asked to log on type in your name this is now your configuration and any subsequent changes you make to the program will be store as defaults under this name individual names and their corresponding user preferences can be added for future selections if you have opened imagesource before and entered your name you will be able to select it from the drop-down list press ok to open imagesource imagesource user manual getting started 2-1

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getting started acquiring the image the main imagesource application window will open with the image capture toolbox displayed on its left-hand side camera ready green button view live image this default/standby mode signifies the camera is ready to capture an image automatically click on button or select file new image from camera with the drop down menu for image capture red button freeze image manual camera manipulations can be accomplished in this mode 1 adjust aperture amount of light with the top ring on camera 2 zooming adjustments can be made with the bottom ring 3 once the image is set click the red button to green the image can now be captured automatically imagesource user manual acquiring the image 2-2

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getting started saving the image using single-click icon from imagesource software the acquired image can be exported directly to imageaide software if available for further analysis click the send to imageaide button or from windows file save as dialog box can be opened for image file to be saved 1 select a folder to hold the image file from the save in drop-down list and the file list box below it 2 enter a name for the image file in the file name box 3 press save to save the image with the new name once you have saved the image it can be imported from imageaide or other commercial analysis software refer to separate imagesource manual referenceimagesource.pdf for full software features and usage imagesource user manual saving the image 2-3

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