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imageezetm user manual all possible care has been taken in the preparation of this publication but spectronics corporation accepts no liability for any inaccuracies that may be found spectronics corporation reserves the right to make changes without notice both to this publication and to the product that it describes no part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without the prior permission of the copyright owner published by spectronics corporation 2008 imageeze 2008 manual

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contents imageeze introduction and initial setup 1 introduction 1-1 initial setup 1-2 overview 1-2 connecting the system 1-3 software overview 1-4 imagesource 1-4 imageaide 1-5 summary 1-5 getting started with imageeze 2 starting up 2-1 acquiring the image 2-2 analyzing the image 2-3 imageeze 2008 manual

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introduction and initial setup introduction this manual gives full instructions for setting up imageezetm and provides basic operations of imagesourcetm acquisition and imageaidetm analysis software the manual is divided into two parts introduction and initial setup and getting started with imageeze initial setup gives a brief overview of the imageeze hardware and instructions for connecting up its components getting started part of the manual takes you through basic procedures for capturing an image and sending it to imageaide for analysis imageeze 2008 manual 3 introduction 1-1

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imageeze initial setup overview imageeze is an affordable fully automated gel documentation and analysis system it features a laboratory grade 8-bit ccd performance with many outstanding features usually only associated with advanced systems standard imageeze system gl-3001 is supplied with · geneline darkroom hood with ccd bracket gl-1000 with gl-0300 fitted with 8-bit ccd camera gl-0100 ccd lens adapter gl-0140 zoom lens gl-0110 close-up lens gl-0120 and standard uv/ir filter gl-2100 · imagesource data acquisition kit gl-3100 which includes acquisition board ccd camera cables attached to camera assembly above and imagesource software on cd rom · · imageaide band matching analysis software on cd rom gl-3300 and security dongle key manuals on cd rom · · · · imageeze.pdf this installation guide referenceimagesource.pdf gettingstartedimageaide.pdf referenceimageaide.pdf imageeze plus system gl-3051 is supplied with · · standard imageeze system components see listing above uv transilluminator 312nm variable-intensity tvc-312r note imageeze must be used with a pc that has windows 98se 2000/xp or vistax86 platform cpu must also have a spare pci slot for installation of prymo card imageeze 2008 manual 4 overview 1-2

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imageeze initial setup connecting the system 1 turn on the pc customer-supplied and load the imagesource analysis software into cd-rom drive 2 the installation program will start automatically if it does not locate the cd rom drive in windows explorer and double-click the setup.exe follow instructions on screen and complete the software installation 3 turn off computer follow normal shut down procedures remove the cover and locate spare pci slot fit the acquisition board into slot appropriately 4 replace the cover of the computer and connect peripheral computer components monitor keyboard mouse etc 5 with hood placed securely on uv transilluminator tvc-312r or your own connect the ccd camera assembly pre-focused at factory to the pc by inserting the serial end of camera cable to the acquisition board serial port on the computer 6 turn on the computer and restart the imagesource software message of successful installation will be indicated 7 load the imageaide analysis software into cd-rom drive follow installation instructions on screen 8 imageeze is now ready for use with a transilluminator light source see getting started with imageeze chapter 2 for imageeze operations imageeze 2008 manual 5 connecting the system 1-3

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software overview imagesource acquisition software imagesource program is used to control the hardware to capture images and to process the captured images in particular imagesource offers control of illumination and exposure length while viewing a live image from the camera you can use imagesource to capture images · · · · from a live image as a single frame capture in a series with the same or variable exposures using auto exposure software capabilities the patented extended dynamic range edr feature allows you to extend the dynamic range of the system s camera in particular it increases the detail in low intensity areas of the image allowing you and analysis software like imageaide to distinguish features in dark parts of the object that would otherwise be undetectable without saturating the lighter areas the neutral field correction provided by imagesource allows you to correct for uneven illumination while maintaining good laboratory practice the histogram display allows you to maximize the image contrast without losing any detail for presentation purposes you can process captured images to create a negative image and increase or decrease the sharpness you can also crop imageeze 2008 manual 6 imagesource software overview 1-5

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software overview images or flip them horizontally or vertically imagesource also allows you to add graphical and textual annotations then save as separate file from the image for further course of action all processing is performed on a copy of the original image which is saved with the original image in accordance with good laboratory practice imageaide will always analyze the original unprocessed image in the image file original and processed images can be exported to files using a wide range of standard graphics formats and you can choose whether or not to `engrave the annotations on the exported image imageaide analysis software imageaide is an extremely powerful and easy-to-use image analysis program it is fully automated allowing analyses to be carried out in seconds however it is also extremely flexible giving you the option to control every aspect of the analysis manually if you wish summary your imageeze system should now be fully assembled and ready for use you should now go to the getting started chapter to find out how to turn it on and start working productively with it imageeze 2008 manual 7 imageaide software overview 1-5

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getting started with imageeze this chapter gives a quick introduction to using your imageeze system taking you through the processes of starting up capturing an image and opening the image in imageaide for analysis starting up 1 make sure the power leads from all components are connected to a live supply 2 place sample on the surface of the transilluminator uv or white light source toward the middle of the viewing field gently position the camera/hood assembly on top of the transilluminator 3 turn on the main power switches for transilluminator computer monitor and printer if applicable the imageeze will take a little time to start up while it loads windows and then imagesource when imagesource starts up the first time you will be asked to log on type in your name this is now your configuration and any subsequent changes you make to the program will be store as defaults under this name individual names and their corresponding user preferences can be added for future selections if you have opened imagesource before and entered your name you will be able to select it from the drop-down list press ok to open imagesource imageeze 2008 manual 8 starting up 2-1

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getting started acquiring the image the main imagesource application window will open with the image capture toolbox displayed on its left-hand side camera ready green button view live image this default/standby mode signifies the camera is ready to capture an image automatically click on button or select file new image from camera with the drop down menu for image capture red button freeze image manual camera manipulations can be accomplished in this mode 1 adjust aperture amount of light with the top ring on camera 2 zooming adjustments can be made with the bottom ring 3 once the image is set click the red button to green the image can now be captured automatically refer to separate imagesource manual for full software usage referenceimagesource.pdf imageeze 2008 manual 9 acquiring the image 2-2

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getting started analyzing the image using single-click icon from imagesource software the acquired image can be exported directly to imageaide software for further analysis to start up imageaide to analyze the image in the selected image window click the send to imageaide button a standard windows save as dialog box will open for image file to be saved 1 select a folder to hold the image file from the save in drop-down list and the file list box below it 2 enter a name for the image file in the file name box 3 press save to save the image with the new name once you have saved the image imageaide will open with the selected image ready for analysis ­ refer to separate imageaide manuals for full software usage gettingstartedimageaide.pdf and referenceimagesource.pdf imageeze 2008 manual 10 analyzing the image 2-3

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