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imagesci and imagesource user manual all possible care has been taken in the preparation of this publication but spectronics corporation accepts no liability for any inaccuracies that may be found spectronics corporation reserves the right to make changes without notice both to this publication and to the product that it describes no part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means electronic mechanical photocopying recording or otherwise without the prior permission of the copyright owner published by spectronics corporation © synoptics limited 2004[close]
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spectronics corporation 959 brush hollow road p box .o westbury new york 11590 tel 1-800-274-8888 fax 1-800-491-6868 website www.spectroline.com[close]
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contents introduction and initial setup introduction imagesci overview connecting up software overview imagesource imageaide summary the system 1 1-1 1-2 1-2 1-2 1-3 1-3 1-4 1-4 getting started with imagesci 2 switching on 2-1 capturing an image from the camera 2-3 inserting the sample 2-3 capturing an image using automatic exposure 2-4 capturing an image from live video 2-5 capturing a single image with an exposure longer than five seconds 2-7 capturing a series of images 2-8 capturing an image with extended dynamic range 2-11 analyzing the image 2-11 hints 2-12 starting imagesource 2-12 logging in 2-13 displaying the image capture toolbox 2-13 image display 2-13 saturation detection 2-13 moving on 2-15 using imagesource 3 working with configurations 3-1 imagesci and imagesource user manual contents-1[close]
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contents user and system default configurations 3-1 selecting a configuration 3-2 creating and saving configurations 3-2 deleting a configuration 3-3 promoting a user configuration 3-4 capturing images from the camera 3-5 capturing an image from live video 3-6 capturing a single image 3-6 capturing images using series capture 3-7 capturing images using auto exposure 3-10 using edr extended dynamic range 3-10 using neutral field correction nf 3-11 adjusting display conditions 3-15 using the image window display controls 3-15 using the histogram window to control the image display 3-16 saving and loading image files 3-19 loading image files 3-20 creating a composite image multiplexing 3-22 manipulating images 3-24 image toolbar 3-25 original and processed images 3-26 sharpening and smoothing images 3-26 inverting images 3-27 embossing an image 3-27 flipping an image 3-27 cropping an image 3-28 placing annotations on an image 3-30 annotate toolbar 3-30 showing and hiding annotations 3-30 selecting the default color and line style for annotations 3-31 drawing a line 3-31 drawing a rectangle 3-32 drawing an ellipse 3-33 drawing a freehand shape 3-35 placing text on an image 3-39 drawing an arrow 3-41 changing annotations 3-43 exporting images and engraving saving and merging annotations 3-46 contents-2 imagesci and imagesource user manual[close]
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contents the imagesource application window 4 status bar 4-2 image capture toolbox 4-3 docking and floating the image capture toolbox 4-4 drawer icon 4-4 freeze/go live/snap image 4-5 open drawer 4-6 exposure time 4-6 automatic exposure 4-7 image series 4-7 lighting control 4-7 extended dynamic range edr control 4-8 neutral field correction nf control 4-9 image windows 4-9 zooming and scrolling image windows 4-10 image controls 4-13 three-dimensional view 4-14 original and processed view modes 4-15 the histogram window 4-16 showing and hiding the histogram window 4-16 docking and floating the histogram window 4-17 using the histogram window 4-18 histogram window cursors 4-19 browser 4-20 toolbars displaying and hiding arranging toolbars image sharpen smooth speckle correction invert flip horizontal flip vertical define region crop to region toolbars 5 5-1 5-2 5-2 5-2 5-2 5-2 5-3 5-3 5-3 5-3 5-3 imagesci and imagesource user manual contents-3[close]
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contents annotate select draw line draw rectangle draw ellipse draw freehand shape draw text draw arrow hollow/filled shapes color line style zoom zoom in zoom out zoom 1:1 zoom to fit standard new open save print image capture toolbox annotation toolbars image toolbar send to imageaide show histogram window 3d view context-sensitive help configuration 5-4 5-4 5-4 5-4 5-5 5-5 5-5 5-5 5-5 5-6 5-6 5-6 5-6 5-6 5-7 5-7 5-7 5-7 5-7 5-7 5-8 5-8 5-8 5-8 5-8 5-9 5-9 5-9 5-9 menus file menu new image from camera new image from file open image browse save image 6 6-1 6-1 6-2 6-3 6-4 6-5 contents-4 imagesci and imagesource user manual[close]
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contents save image as 6-5 close image 6-6 export image 6-7 merge annotation 6-8 create new composite image multiplexing 6-9 printer setup 6-12 report settings 6-13 print preview 6-13 print 6-14 most recently used files 6-15 exit 6-15 edit 6-16 cut 6-16 copy 6-16 paste 6-16 undo 6-16 view 6-17 annotation 6-17 histogram 6-18 grid on live 6-18 normal 1:1 6-18 zoom in 6-18 zoom out 6-19 view original 6-19 view processed 6-19 properties 6-20 status bar 6-23 3d view 6-24 window 6-24 cascade 6-24 tile horizontal 6-24 tile vertical 6-24 arrange icons 6-25 close all 6-25 windows open in imagesource 6-25 tools 6-25 image 6-26 annotate 6-26 imagesci and imagesource user manual contents-5[close]
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contents zoom standard extras user name report titles initial acquire state save configuration delete configuration promote configuration autoscale on capture image sharpen smooth invert flip horizontal flip vertical emboss define region of interest crop to region help contents index using help show wizard change wizard about imagesource 6-26 6-27 6-27 6-27 6-28 6-29 6-30 6-31 6-31 6-33 6-33 6-33 6-34 6-35 6-35 6-35 6-36 6-37 6-38 6-38 6-38 6-39 6-39 6-39 6-39 6-40 contents-6 imagesci and imagesource user manual[close]
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introduction and initial setup introduction this manual gives full instructions for setting up and using imagesci and for using the imagesource software the manual is divided into three parts introduction and initial setup this chapter getting started and reference the next section in this chapter gives a brief overview of the imagesci hardware and instructions for connecting up its components the final section of the chapter gives an overview of the software the getting started part of the manual takes you through basic procedures for capturing an image and sending it to imageaide for analysis the first chapter in the reference part of the manual chapter 3 using imagesource describes how to use setting configurations capture images and control the way images are displayed it then shows you how to work with captured images for presentation purposes including how to enhance them using a variety of techniques and how to add annotations chapter 4 the imagesource application window describes the layout of the program interface and shows you how to work with the image capture toolbox image windows the histogram window and the browser chapter 5 the toolbars describes some general features of working with toolbars and then provides instructions for using each of the tools chapter 6 menus describes each of the menus in turn with instructions for using the commands they contain imagesci and imagesource user manual 1-1[close]
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introduction and initial setup imagesci overview imagesci is an affordable fully-automated gel documentation and analysis system it features 16 bit performance with many outstanding features usually only associated with advanced systems standard imagesci systems are supplied with imagesci darkroom fitted with 8-bit 16-bit performance provided by edr ccd camera and prefocused f1.2 lens with standard uv filter integral transilluminator 15 x 15cm 302nm sample size 15 x 12cm uv/white light converter for white light applications optional built-in pc running windows xp 250mb internal zip and 1.44mb floppy drive imagesource acquisition software imageaide match analysis software microsoft office small business edition optional imagesci is supplied with all necessary software installed in the pc so all you need to do is to connect a keyboard mouse and monitor connecting up the system to connect up imagesci 1 unscrew the knurled fixing screw holding the cover plate on the left-hand side of the imagesci system box remove the cover plate to reveal the connectors beneath plug in the monitor keyboard and mouse leads the sockets are labelled with appropriate icons 2 3 note you should also connect any other accessories such as a printer to the appropriate connectors beneath the cover panel 1-2 imagesci and imagesource user manual[close]
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software overview 4 refit the cover panel slotting the cables through the cutouts and replace the knurled fixing screw to hold it in place connect power cables to the power sockets on the imagesci on the back and monitor 5 see getting started with imagesci chapter 2 for how to switch on the imagesci and use it to capture images software overview this section gives an overview of the software supplied with imagesci imagesource you use the imagesource program to control the hardware to capture images and to process the captured images in particular you can use imagesource to control the illumination and exposure length while viewing a live image from the camera you can use imagesource to capture images from a live image as a single frame capture in a series with the same or variable exposures using auto exposure the patented extended dynamic range edr feature allows you to extend the dynamic range of the system s camera in particular it increases the detail in low intensity areas of the image allowing you and analysis software like imageaide to distinguish features in dark parts of the object that would otherwise be undetectable without saturating the lighter areas the neutral field correction provided by imagesource allows you to correct for uneven illumination while maintaining good laboratory practice a histogram display allows you to maximize the image contrast without losing any detail for presentation purposes you can process captured images to create a negative image and increase or decrease the sharpness you can also crop images or flip them horizontally or vertically imagesource also allows you to add graphical and textual imagesci and imagesource user manual 1-3[close]
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introduction and initial setup annotations you can save the annotations in a separate file from the image to transfer them to other images all processing is performed on a copy of the original image which is saved with the original image in accordance with good laboratory practice imageaide always analyses the original unprocessed image in the image file original and processed images can be exported to files using a wide range of standard graphics formats and you can choose whether or not to `engrave the annotations on the exported image imageaide imageaide is an extremely powerful and easy-to-use gel analysis program it is fully automated allowing analyses to be carried out in seconds however it is also extremely flexible giving you the option to control every aspect of the analysis manually if you wish see the separate imageaide documentation for details summary your imagesci system should now be fully assembled and ready for use you should now go to the getting started chapter to find out how to turn it on and start working productively with it the first chapter in the reference part of the manual using imagesource builds on the getting started chapters and goes into rather more depth showing you alternative ways of proceeding and more advanced features the remaining chapters in the reference part of the manual provide a systematic reference to the imagesource software 1-4 imagesci and imagesource user manual[close]
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