p. 1

image documentation products

[close]

p. 2

genelinetm image documentation products background gel documentation products offered by spectronics under the brand name genelinetm allow the end-user to acquire view transfer archive and analyze the gel images all in real-time and all on a digital pc format · product lines imageezetm · 8-bit ccd camera with enhanced pixel resolution compact darkroom hood pci interface card for use with user-supplied pc imagesourcetm acquisition kit imageaidetm band matching analysis software our component-based series imageezetm offer end-users fully automated gel documentation and analysis products our geneline systems and components combine darkroom hoods for transillumination or cabinets transillumination and epillumination cameras image acquisition software and analysis software to provide consistently high quality gel images the acquisition software imagesourcetm or digital camera solution disk acquires images allows further manipulation and saves the images all with computer-control for ultimate ease the imageaidetm analysis software receives digitized image directly at the touch of a button or can alternatively import any picture-formatted document sgd bmp jpg tif extensions imageaidetm performs a complete auto-analysis and quantification of images including band matching instantly for those requiring customized systems all geneline components are available separately for mix-and-match packages to best suit your exact application and budget · · · components · ccd camera and acquisition imagesource kit provide edr 16bit image acquisition fully computer controlled functions includes separate image capture and image analysis software with band matching capability darkroom hood for use with transilluminators fluorescence analysis cabinets with various camera adapters for use with hand-held lamps and transilluminators · · · · 2

[close]

p. 3

imageezetm hood style system · features laboratory grade 8-bit ccd camera with edr 16-bit acquisition · · · ultimate simplicity in image acquisition analysis and reporting one button transfer of acquired image to analysis software gels blots and colony images are easily acquired and analyzed · plus package available with transilluminator imageeze is a complete image acquisition and analysis gel documentation system this component system features an 8-bit ccd camera with enhanced pixel resolution computer interface pci card imagesource image acquisition software and imageaide band matching analysis software the system offers a direct method to acquire view real time control of images with automatic exposure and zooming capabilities archive transfer and analyze gel images imagesource software provides enhanced 16-bit extended dynamic range edr image acquisition capability to the cost-effective 8-bit ccd camera to prevent loss of image information one button click eases transfer of image data to imageaide analysis software for sophisticated icon-driven analyses of all types of gel and spot images using imageeze is like using an ordinary camera providing the ultimate point-and-shoot system simply place the darkroom hood-mounted camera on the transilluminator over the gel to be photographed and the image is seen immediately on the computer monitor in real time make minor adjustments to the camera aperture and then click the auto-exposure control the camera automatically adjusts to produce the ideal image on the computer screen you can then simply save the image file archives with default sgd extension then send it to imageaide for further image analysis and see virtually all the data and results displayed in one window also available imageeze plus imageeze system with tvc-312r variable intensity transilluminator 15w 312nm 20x20cm filter imageeze specifications camera lens filter assembly images camera hood acquisition software band matching analysis software computer user provided 8-bit ccd with on-chip integration 8-48mm zoom +2 diopter lens and uv filter 768 x 494 pixels fits over 21cm x 26cm samples imagesource for automated image capture imageaide for gels colonies spots blots manual band quantification operating platforms windows 2000/xp 98se models gl-3001 gl-3051 plus camera kit gl-4500a gl-4500a hood gl-1000 gl-1000 acquisition gl-3100 gl-3100 analysis gl-3300 gl-3300 transilluminator user provided tvc-312r 3

[close]

p. 4

imageezetm cabinet style components · offers all the features of the imageeze system with additional epiillumination option · various sized fluorescence analysis cabinets with and without white light · · add appropriately sized spectroline uv lamps for epi-illumination add spectroline uv/white light transilluminator for transillumination · all components including imageaide analysis software are available separately those requiring sample illuminations from above and below our cost-effective geneline components offer epiand trans-illumination capabilities with one complete cabinet-style system components include ccd-based camera imagesource acquisition kit and imageaide band matching analysis software coupled to our laboratory transilluminators cabinets and uv lamps for epi-illumination of samples our selection of fluorescence analysis cabinets include cc-80 for use with our eseries 4 6-watt lamps cl-150 for use with our x-series 15-watt lamps and cm-10mp for use with our minimax 4/5-watt mini-lamps all our uv lamps are available in uv short medium and long wavelength combinations please refer to our website www.spectroline.com for our complete product line for acquisition the ccd camera is attached to a camera adapter with metal bracket and snapped onto cc-80 or cl-150 cabinet images are then viewed and acquired with imagesource and further analyzed with imageaide band matching software specifications for fluorescence analysis cabinets · · · · · · dual lamp ports for adding or interchanging with other spectroline uv lamps flexible contoured eyepiece includes uv-absorbing window for viewing uv without additional eyewear removable eyepiece allow substitution of other custom `snap-on spectroline camera adapters felt curtain permits easy interior access while blocking out ambient light visible epi-illumination source provided with internal 25 watt white light bulb external light switches on top of cabinet allow individual controls of all light sources including internal white light and plugged in uv lamps rugged construction of aluminum housing with chemical-resistant coating · cc-80 · · for use with e-series 8-watt uv lamps · housing dimension lxwxh 20.25 x 17.5 x 9.25 51.4 x 44.5 x 24.8cm weight 11.5 lbs 5.2 kg cl-150 · · for use with x-series 15 watt uv lamps · housing dimension lxwxh 19.5 x 24.25 x 12.75 49.5 x 61.6 x 32.4cm weight 15.0 lbs 6.8 kg 4

[close]

p. 5

mini-epicamtm components · mini image documentation system with all the features of our larger geneline systems · cost-effective with optional minimaxtm uv lamps for epiillumination · add a spectroline slimlinetm transilluminator shown here for trans-illumination developed specifically for acquiring images of small gels and fluorescent samples mini-epicam components provide all the capabilities of our larger geneline series allowing both epi and transilluminations with our small footprint cm-10mp fluorescence analysis mini-cabinet for greater flexibility and cost-effectiveness numerous minimaxtm uva and/or uvc 4 and 5 watt uv tube lamps are available separately for use with the cm-10mp please refer to our minimax uv lamp flyer or visit our website www.spectroline.com for details and models specifications for cm-10mp mini-cabinet · · · · · · dual lamp ports for adding spectroline minimax 4 and 5-watt uv lamps flexible contoured eyepiece includes uv-absorbing window for direct viewing removable eyepiece for easy substitution of spectroline gl-1301 adapter lightweight plastic construction for easy positioning over sample housing dimension lxwxh 8.0 x 8.25 x 6.0 20.3 x 23.5 x 21.0cm weight 1.85 lbs 0.84 kg specifications for gl-1301 digital camera adapter for cm-10mp · · · · · sturdy painted sheet metal housing with rubber o-ring custom height for use with gl-4200b digital camera outfitted with 58mm camera lens ring for easy camera assembly attachment housing dimension lxwxh 3.75 x 8.25 x 6.0 9.5 x 21.0 x 15.2cm weight 1.55 lbs 290g specifications for uv-5a minimax uv lamp · · · · · cost-effective 5-watt long wave uv-a tube lamp versatile battery-operation 4xaa or with ac plug included lightweight plastic polymer housing dimension lxwxh 9.0 x 2.25 x 1.0 22.9 x 5.7 x 2.5cm weight 10.25 oz 290g 5

[close]

p. 6

imagesourcetm image acquisition software · provides fully automated control of ccd camera functions for both focusing and acquisition · on-screen display of system status · real-time live image positioning and manipulation · icon-driven single-click control of software capabilities · direct link to imageaide analysis software · saves images in wide range of file formats imagesource software package was developed for ultimate ease of use while manipulating digitized images the software is perfect for acquiring annotating and printing of laboratory images utilizing a simple-to-use interface offering a wide range of icon-based functions imagesource provides the ultimate image management imagesource is available as an acquisition constituent of imageeze system or sold separately as imagesource data acquisition kit gl-3100 the kit includes the interface card acquisition board ccd camera cables and imagesource software software application window and toolbars image capture toolbox offers user-friendly configuration controls for extended dynamic range edr and neutral field correction nf capabilities include controls for live/freeze/snap capture camera integration saturation detection for quantitative applications brightness/contrast/gamma zooming/scrolling and 3-d view image acquisition features · using automatic or manual exposure · from live video · with an exposure longer than five seconds · capturing series of images · capturing with edr or nf fully automated controls of · camera functions · image displays and manipulations · saturation detection · histogram distribution · editing functions including multiplexing 6

[close]

p. 7

annotate image toolbar offers cropping drawing and placing annotations in the image text lines arrows and shapes can be added in different colors and sizes the annotations are embossed and stored separately from the original image this processed image can then be exported to a standard graphics file with the annotations `engraved over the original image enhance image toolbar offers defining and cropping of image regions boundary sharpening and smoothing image speckle correction and inversion additionally images can be flipped horizontally or vertically as desired 7

[close]

p. 8

additional software features· user defined system configurations for creating and saving default image settings imageeze series · complete help section located in standard toolbar is accessible anytime during use of imagesource · file menu for acquiring importing and manipulating previously saved images · · · includes full windows-based document functions such as cut copy paste and undo report and printing settings are also accessible single-click icon to send image directly to imageaide for analysis periodic software updates are provided free of charge to original purchasers of imagesource software 8

[close]

p. 9

imageaidetm band matching image analysis software · · · · · · · icon-based software for ultimate simplicity to provide complete and accurate analysis instantly auto track band peak lane and edge finding even on indistinct bands track profile comparison band matching features with applicable dendogram data integral mw libraries and user-defined quantification units ng pg or glp compliant software including reporting direct exporting of results to excel and word computer user-supplied imageaide is a software package used for analyzing and quantifying 1-d gel 2-d colony and spot images it is the easiest and the most efficient way to analyze all types of gels multi-tier protein gfp pcr coomassie blue and silver-stain autorad slot blot and colony plates secured sgd default images from ccd and digital cameras are imported directly into imageaide or non-secured jpg bmp tif images can be imported simply select ok for a complete fully automated analysis of the image instantly imageaide will automatically identify and display lanes bands edges then produce track profiles and results all on one window the software includes an extensive library of molecular weight markers to choose from or the molecular weight for each band can be manually added imageaide a major module of all geneline imaging systems is the analysis constituent of our imageeze series the software is also sold separately as imageaide analysis software gl-3300 periodic software updates are provided free of charge to original purchasers of imageaide software 9

[close]

p. 10

features detailed parameters to promote user friendliness individuals can save their own preferred parameters configuration set-up making subsequent analysis even faster imageaide recognizes individual users under user name list and their selected parameters each time they log into the software when imageaide is logged on subsequently the software will default to the last user 10

[close]

p. 11

when an image is loaded all aspects of the image are accessible with one window under sample properties see examples of gel and spot blot below · · general tab displays automated image information that can be edited by user details below information tab displays data saved with the image when the sample file was created through imagesource or another acquisition software · acquisition notes tab displays user notes made and saved with image file in imagesource acquisition software · analysis notes tab displays notes to be edited by user and saved with the secure sample file in imageaide analysis software selections of image analysis types include colony gel manual band quantification and spot blot accessible with analysis type drop down menu once type is chosen all other applicable features are automatically turned on for further selection and qualification image preview pane general tab when a new image is imported the imageaide offers users complete control over application type and analysis of the image the image preview pane defines the area of interest for analysis by default imageaide will only locate tracks or spots contained within the red box user can adjust as necessary for customizing image selection prior to further detailed analysis additionally imageaide will automatically analyze and classify all types of gel or spot media features of automated analysis include gel type recognition of either fluorescence or absorption shown under image type and track direction under electrophoresis direction imageaide features both manual track selection or auto track location under tracks in auto mode a single button operation not only locates all tracks on a gel but also goes on to complete a full analysis clicking on ok sends the image for further detailed automated analysis the autotrack finding feature also detects distorted or misshapen tracks and bands 11

[close]

p. 12

fast analysis of gel images selected image from sample properties is imported instantly into the quantitative portion of imageaide here with one gel window view lanes bands edges and track profiles are automatically calculated and raw data in both table and graph formats are displayed for further review and analysis all available image manipulation features are accessible through drop-down menus or corresponding icon selections perfect for those with limited english gel window image paneshows image with markers curved to match the data track label paneshows the number of each track and the type when a calibration is selected profile paneshows the profile of each peak to include positions and boundaries description paneshows entered info for selected track peak value paneshows data for the peaks shown in the profile pane reset brightness brightness adjustment reset contrast contrast adjustment reset gamma gamma adjustment graphics pane results pane 12

[close]

p. 13

track editing a truly useful function is track editing unlocking band manipulation allows individual tracks to be deleted moved or adjusted for width and position locking prevents unwanted manipulation of information during analysis manual compensation for curved tracks and distorted gels through rf changes starting ending and bending is also possible track location lock unlock position splay tracks move tilt width rf positions start end calibration in order to determine molecular weights or quantities of unknown bands in selected sample tracks calibration of a reference track to standardized molecular weights or quantities must first be made a library of standards for molecular weight is included with imageaide it can also be easily edited to include new markers and manual weights quantity calibration can be either manually or with automatically assigned quantities quantity assignment mw selection once reference track is assigned with chosen molecular weight or quantities for comparison all outlined bands in other selected tracks are automatically quantified and information is displayed in one window format 13

[close]

p. 14

band matching for imageaide band matching analysis software peaks on sample tracks can be matched to peaks on a standard or different track this can be accomplished through either auto match peaks on the general page of imageaide configuration dialog or peak match menu or icon peak matching icons cross gel track profiling analyzed tracks on different tracks and/or different gels saved with the imageaide software can be opened displayed and overlaid for profile comparison choose profile comparison from menu or click on icon for comparison window to open profile comparison a profile comparison analysis can be customized through configuration menu adding references and changing the scales as necessary additionally reports of profile comparisons can also be viewed and printed 14

[close]

p. 15

fast analysis of colonies like gel images colony plate images sgd bmp jpg tif can be imported for further analysis sample properties dialog box will open for general information acquisition and analysis notes tabs imageaide automatically detects the image type but setting of light and dark colonies can be made manually by defining the background standard quantity by which colonies are defined lighter or darker than set background colony counting window the results box shows the number of colonies counted this process is entirely automatic but individual colonies can be manually added or removed for the automatic count areas to be counted can be set under define frame and parameters controlling the way they are counted can also be set under auto detection/sensitivity after selecting show colony shapes box or using configuration colonies can also be divided into classes as defined by their image density under separate colonies area limits colony size inclusions and exclusions are selected with colony counting area limits dialog box whereby minimum and maximum colony areas are characterized the results box is updated to show changes in colony counting affected by changes in detection parameters and defined regions moving or reshaping the counting frame 15

[close]

p. 16

fast analyses of manual band quantification and spot blot images imageaide also has options for automatic analyses of manual band quantification spot blots and arrays like gel images spot/band images sgd bmp jpg tif can be imported for further analysis sample properties dialog box will open for general information acquisition and analysis notes tabs the analysis type will probably be set to gel when the dialog box opens initial default but selection of manual band quantification or spot blot can be made with drop-down menu imageaide automatically detects the image type fluorescence or absorption spot blot/manual band quantification window when imageaide analyzes a manual band quantification image it takes measurements from defined areas boxes drawn by end-user for spot blot image it takes measurements from a defined spot spot frame for image analysis rectangular circular or free hand shapes can be placed on bands while automatic or manual grids can be placed over the spots spot blot image pane results/incidence/calibration graph pane spot/band editing like track editing features bands and spots can be automatically or manually analyzed manual compensation for unusual regions is accomplished with unlocking spot/band image allowing individual regions to be deleted moved or adjusted for size and position additional features including quantity assignment and background correction are also available spot/band positioning locate background correction spot incidence drawing/defining region assigning quantity when assigning quantity to a spot frame or manual band box to calibrate quantity measurements the selected region is marked with a cross 16

[close]

p. 17

results configurable results are a feature of imageaide for gel analysis graphs of mw calibration and quantity calibration are shown band matching software displays an additional dendrogram pane spreadsheets showing raw data such as peak height area volume band number and position band matching software displays additional matching comparisons matrix and coefficient tables the software also has its own glp reporting system for ultimate security and compliance for colony counting results include histogram of the light and dark colonies as well as raw data for both classes of colonies for spot blot and manual band quantification results table can be customized based on the amount of information to be viewed and compared right click on results section or select through menu to add data to table 17

[close]

p. 18

reporting imageaide has the most comprehensive reporting system for a package of its type every detail of the sample results date and time are recorded in defaulted glp formatted report these reports are configurable under report setup so they can be tailored for the needs of each user for greater options analysis information can be exported to word for documentation and incorporation into papers additionally data tables can be exported to excel for further detailing and charting the report toolbar below shows all the reporting options and is displayed when any sample window or the profile comparison window is selected setup preview print export to excel save to csv file export save to to word clipboard sample report pages for gel analysis 18

[close]

p. 19

using imageaide for image analysis imageaide provides a range of tools to give you fine control carrying out procedures manually or for editing the results of its automatic operations however you will probably only need to use them very occasionally since imageaide s automatic tools are so powerful and sensitive that they will handle all but most exceptional analyses toolbars standard open save help sample properties report setup preview print export table t0 excel save table to csv file export to word save to clipboard track locate lock/unlock position splay move/tilt width rf positions zoom in out reset calibrations integration parameters quantity calibration assign quantity molecular weight calibration mw standard profile comparison window peak matching of selected bands spot blot size position locate background correction lock/unlock drawing quantity calibration gel analysis create and work with secure sample files working with tracks place position and work with tracks set background correction for tracks locate and edit peaks on tracks create and use molecular weight calibrations create and use quantity calibrations compare peak profiles peak matching on bands print and export gel results colony count analysis create and work with secure sample files working with colony counting windows defining counting region setting detection parameters viewing and manipulating colonies and class boundary printing colony count results manual band quantification create and work with secure sample files working with boxes on samples placing rectangular or free hand box adjust size shape and position of boxes utilizing identification labels on boxes set and assign quantity calibration method define incidence condition viewing incidence matrix print and export manual band quantification results spot blot analysis create and work with secure sample files working with spot frames adjust size shape and position of spot frames utilizing identification labels view and manipulate spot blot results set and assign quantity calibration method define incidence condition print and export spot blot results 19

[close]

p. 20

geneline components software and accessories ccd camera kit gl-4500a includes 8-bit ccd camera gl-0100 with zoom lens gl-0110 lens adapter gl-1040 close-up lens gl-0120 uv filter gl-2100 and ccd camera bracket gl-0300 imagesource acquisition kit gl-3100 includes acquisition board camera cables and software imageaide analysis software gl-3300 band matching analysis software with dongle key replacement parts gl-0100 laboratory grade 8-bit ccd camera without cables gl-0110 zoom lens for gl-0100 8-48mm gl-0120 close-up lens +2 diopter 58mm ring gl-0140 lens adapter for gl-4500a ccd camera 58mm ring gl-2100 uv filter 58mm ring hoods and adapters geneline darkroom hood painted sheet metal housing with bottom rubber o-ring for 21x26cm samples hood dimension l xwxh 9.75 x 10.75 x 16 24.8 x 27.3 x 40.6cm gl-1000 replacement hood without camera bracket gl-0300 ccd camera bracket bracket dimension w xhxd 31/8 x 57/8 x 3/4 7.9 x 14.9 x 1.9cm camera adapters for photo documentation cabinets painted sheet metal housing with snap-on metal pins gl-1100 replacement camera adapter for cc-80 without camera bracket dimension l xwxh 8 x 6.5 x 8 20.3 x 16.5 x 20.3cm gl-1200 replacement camera adapter for cl-150 without camera bracket dimension l xwxh 5 x 6.5 x 8 12.7 x 16.5 x 20.3cm painted sheet metal housing with bottom rubber o-ring gl-1300 replacement camera adapter for cm-10mp without camera bracket dimension l xwxh 3.75 x 8.25 x 6 9.5 x 21.0 x 15.2cm 20

[close]