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genomed 2012/2013

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© 2013 genomed gmbh all rights reserved genomed gmbh poststraße 22 32584 löhne germany tel +49 0 57 32 90 47 00 fax +49 0 57 32 9 04 70 10 web page www.genomed-dna.com e-mail service techservice@genomed-dna.com bluescript is a registrated trademark of stratagene inc dye terminator is a trademark of applied biosystems sequenase is a registrated trademark of united states biochemicals triton is a registrated trademark of rohm haas the polymerase chain reaction pcr is covered by patents owned by hoffmann-la roche ag.

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contents introduction 2 technologies techniques 4 plasmid purification kits 7 gel extraction kits 27 pcr product purification kits 33 general dna clean up kits 37 blood cell culture dna purification kits 41 tissue dna purification kits 49 accessories equipment 52 product list 54 distributors 56 www.genomed-dna.com 1

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introduction who we are genomed is a german company founded 1991 founder of genomed is a former qiagen r&d employee who developed the first ever ready-to-use plasmid kits on the basis of an anion exchange resin these kits were launched by qiagen in 1986 genomed was founded to develop and market products to isolate and purify nucleic acids dna purification kits in particular are the interest of genomed our short term and long term goals are the development and use of innovative technologies to make the daily work in biological labs easier simpler and more convenient we focus on high quality products exclusively and thus all products are manufactured and quality controlled at the genomed facility in löhne where we are the company is located in löhne a medium-sized town in the north of germany hannover and its international airport is about 40 miles away how we sell our products genomed distributes all products under its own label around the world germany and austria are supplied from the german facility of genomed directly in foreign countries genomed is in contract with distributors who are selling the products under our label additionally genomed has some oem contracts with qualified biotechnology companies selling the genomed products under their own label patents all genomed products are developed by genomed itself our intellectual properties are protected by pending patents worldwide 2 www.genomed-dna.com

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the map the building the lab www.genomed-dna.com 3

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genomed iex resin technologies techniques description the genomed anion exchange resin iex was developed in the early 90´s and became the core element of genomed´s jetstar plasmid purification kits in 1994 the jetstar kits are designed to achieve ultrapure plasmid and cosmid dna from e.coli cells which has biological activity equivalent to more than two rounds of cscl gradient purification our experience over the last six years indicates that jetstar purified dna is ideal for applications like automated sequencing transfection microinjection and gene therapy research applied in gravity flow columns iex resin specifications anion exchange chromatography silica based resin silica beads with uniform particle size extraordinary large surface because of very small pores ultra high charge density because of the large surface ultra high charge density provides very high dna capacity surface coated with positively charged deae groups deae groups are linked to resin by very long spacers long spacers provide an excellent separation range gravity flow columns are available in mini midi and maxi vacuum cartridges are available in mega and giga format porous solid-phase or vacuum cartridges elution profile of the jetstar iex resin associated procedure lysis of bacteria phenol/chloroform extraction no organic or toxic reagents gravity flow column procedure or vacuum cartridge procedure plasmid dna of all sizes can be prepared plasmid dna recovery is 90-95 on average a260/280 ratio is 1.85 endotoxin level is 0.1 eu/µg plasmid dna sds/naoh no associated products plasmid purification kits mini midi maxi jetstar plasmid purification kits mega giga jetstar/lfu plasmid purification kits midi maxi no endo jetstar endotoxin-free plasmid purification kits maxi mega giga jetstar 4 www.genomed-dna.com

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genomed membranes description the genomed silica membranes are non-charged materials that bind dna within defined parameters over the last years genomed has developed a number of different silica membranes which solve individual problems of dna binding and purification the silica membranes were designed for use in different types of spin columns applied in micro spin columns silica membrane specifications based materials individually modified membrane surfaces selective adsorption of dna silica associated procedure centrifugation procedure always following the bind-wash-elute principle no phenol/chloroform extraction no organic or toxic reagents no alcohol precipitation dna binding under high salt conditions dna elution always with te buffer or water a260/280 is usually 1.8 or large spin columns associated products plasmid purification spin kit gel extraction spin kit jetquick pcr product purification spin kit jetquick general dna clean up spin kit jetquick blood cell culture dna spin kit jetquick tissue dna spin kit jetquick jetquick www.genomed-dna.com 5

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genomed silica resins technologies techniques description the genomed silica resins are non-charged high quality spherical silica beads depending on their specific applications the resins are surface modified differently all silica resins are applied as resin suspensions and used in so-called batch procedures jetsorb suspension silica resin specifications silica beads modified resin surfaces selective adsorption of dna spherical individually associated procedure centrifugation no procedure phenol/chloroform extraction no organic or toxic reagents no alcohol precipitation dna elution always with te buffer or water a260/280 is usually 1.8 associated products jetsorb gel extraction kit 6 www.genomed-dna.com

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plasmid purification overview jetstar jetstar lfu plasmid purification products high speed gravity flow columns prep sizes mini midi maxi high speed gravity flow columns including lysate filtration unit lfu prep sizes midi and maxi high speed vacuum cartridges prep sizes mega giga dna purity culture volumes 8 10 12 14 16 noendo jetstar plasmid preps prep sizes maxi mega giga plasmid preps prep sizes advanced mega giga endotoxins 18 20 22 23 24 product list jetquick jetstar products high speed microspin columns prep sizes mini www.genomed-dna.com 7

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overview specifications plasmid purification jetquick mini jetstar mini j etstar midi jetstar maxi jetstar mega jetstar giga spin column mini column midi column maxi column mega cartridge giga cartridge binding-matrix technique vacuum required ready-to-use components phenol/chloroform toxic reagents alcohol precipitation plasmid sizes cosmid dna preparation culture volume high copy pl dna yield high copy pl culture volume low copy pl dna yields low copy pl dna recovery time per preparation plasmid purity a260/280 ratio endotoxins eu/µg dna fluorescent sequencing capillary sequencing radioactive sequencing transfection of sensitive cells microinjection in vitro transcription pcr template preparation restriction analysis library screening ligation cloning labeling membrane spin column column no/optional yes no no no up to 20kb optional 1-5ml up to 40µg 90-95 <20min highly pure 1.80 iex resin gravity flow column no yes no no yes all sizes yes 1-3ml up to 30µg 10-15ml 2-15µg 90-95 40min ultrapure 1.85 0.1 iex resin gravity flow column no yes no no yes all sizes yes 15-25ml up to 150µg 25-100ml 5-100µg 90-95 40-60min ultrapure 1.85 0.1 iex resin gravity flow cartridge no yes no no yes all sizes yes 100ml up to 750µg 250-500ml 50-500µg 90-95 40-60min ultrapure 1.85 0.1 iex resin vacuum cartridge yes yes no no yes all sizes yes 500ml up to 2500µg 2500ml 2500µg 90-95 30min ultrapure 1.85 0.1 iex resin vacuum yes yes no no yes all sizes yes 2500ml up to 10000µg 5000ml 5000µg 90-95 30min ultrapure 1.85 0.1 8 www.genomed-dna.com

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specifications jetstar/lfu midi jetstar/lfu maxi jetstar/jetfilter mega jetstar/jetfilter giga midi column lysate filter maxi column lysate filter mega cartidge lysate filter giga cartridge lysate filter binding-matrix technique vacuum required ready-to-use components phenol/chloroform toxic reagents alcohol precipitation plasmid sizes cosmid dna preparation culture volume high copy pl dna yield high copy pl culture volume low copy pl dna yields low copy pl dna recovery time per preparation plasmid purity a260/280 ratio endotoxins eu/µg dna fluorescent sequencing capillary sequencing radioactive sequencing transfection of sensitive cells microinjection in vitro transcription pcr template preparation restriction analysis library screening ligation cloning labeling sensational results iex resin gravity flow column no yes no no yes all sizes yes 25-50ml up to 150µg 25-100ml 5-100µg 90-95 30min ultrapure 1.85 0.1 optimal results good results iex resin gravity flow column no yes no no yes all sizes yes 100ml up to 750µg 250-500ml 50-500µg 90-95 40min ultrapure 1.85 0.1 sufficient results iex resin vacuum cartridge yes yes no no yes all sizes yes 500ml up to 2500µg 2500ml 2500µg 90-95 20min ultrapure 1.85 0.1 iex resin vacuum cartridge yes yes no no yes all sizes yes 2500ml up to 10000µg 5000ml 5000µg 90-95 30min ultrapure 1.85 0.1 not recommended not known or not designed for www.genomed-dna.com 9

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jetstar 2.0 plasmid purification mini midi maxi specifications jetstar plasmid purification kit patented genomed anion exchange resin iex high speed gravity flow columns preparation of plasmid dna from e.coli cells preparation of cosmid dna and bac dna no chromosomal dna contamination no phenol/chloroform extraction no organic or toxic reagents preparation sizes mini midi maxi technical data gravity flow column mini plasmid dna of all sizes can be prepared plasmid dna is ultrapure better than 2 x cscl plasmid recovery is 90-95 on average total procedure is 40-60 min a260/280 ratio is 1.85 mini prep plasmid yield is up to 30 µg dna midi prep plasmid yield is up to 150 µg dna maxi prep plasmid yield is up to 750 µg dna endotoxin level is 0.1 eu/µg plasmid dna downstream applications gravity flow column midi gravity flow column maxi fluorescent sequencing capillary sequencing radioactive sequencing transfection of endotoxin-sensitive cells microinjection in vitro transcription pcr template preparation all other enzymatic reactions 10 www.genomed-dna.com

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procedure the procedure employs a modified alkaline/sds method to prepare the cleared lysate after neutralization with potassium acetate buffer the lysate is applied onto the jetstar column and the plasmid dna is bound to the patented anion exchange resin jetstar which was developed by genomed one to two washes of the column remove rnas proteins residual endotoxins and all other impurities finally the purified plasmid dna is eluted in high salt sodium chloride buffer from the column and concentrated by an alcohol precipitation the procedure is completed in 40-60 min purification of a plasmid jetstar gravity flow procedure mini midi maxi 1 cleared lysate gel analysis 1 agarose gel of a 3 ml high copy plasmid dna pbluescript iiks preparation before lane 1 from the left and after plasmid purification with the jetstar 2.0 plasmid kit lane 5 the rna is found in the flow-through lane 2 and the washes lanes 3 and 4 2 washes 3 dna elution ligation analysis 4 alcohol precipitation 5 ultrapure dna 0 10 20 30 40 50 60 70 80 90 100 40 60 min total procedure in minutes plasmid dna pbluescript ii ks was prepared using the jetstar 2.0 plasmid kit the plasmid dna was digested with ecori and ligated with a 1.65 kb fragment from the left lane 2 shows the dnas before ligation ligations were set up for 1 hour at 25oc with 0.02 u lane 3 0.05 u lane 4 and 0.1 u lane 5 of t4 dna ligase length standard in lane 1 is the gibco brl 1 kb ladder www.genomed-dna.com 11

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jetstar lfu lysate filtration unit midi and maxi plasmid preparations lysate filtration unit lfu filtration membrane plastic housing inserted into the lysate filtration unit lfu is a fast and convenient device to clear bacterial lysates without centrifugation the lfu is used as a filtration device it removes bacterial debris and sds precipitates completely the lfu fits perfectly into the jetstar ionexchange maxi and the newly designed jetstar ionexchange midi columns the filtration process is done within the jetstar column.lysate filtration and dna binding to the jetstar resin work simultaneously after filtration the lfu is removed and discarded the resin-bound plasmid dna is washed with wash buffer and finally eluted from the column with elution buffer an isopropanol precipitation to collect the dna follows the jetstar/lfu plasmid maxi kit and the jetstar lfu plasmid midi kit come with lfus pre-inserted into the jetstar columns various package sizes are offered see below technical data jetstar/lfu midi maxi midi binding culture culture newjetstar midi column jetstar maxi column maxi iex resin 100 ml 250-500 ml up to 750 µg 50-500 µg 90-95 40 min ultrapure 0.1 1.85 matrix iex resin volume high copy pl 15-50 ml volume low copy pl 50-100 ml high copy pl low copy pl up to 150 µg 5-100 µg 90-95 30 min ultrapure 0.1 1.85 dna yield jetstar lfu midi column jetstar/lfu maxi column dna yield time dna recovery per preparation purity eu/µg dna plasmid endotoxins a260/280 product jetstar/lfu plasmid midi kit 25 jetstar/lfu plasmid midi kit 100 jetstar/lfu plasmid maxi kit 20 jetstar/lfu plasmid maxi kit 100 kit contents preps 25 100 20 100 cat no 211025 211100 221020 221100 jetstar columns lfu buffers enzymes and protocol 12 www.genomed-dna.com

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procedure the lysate filtration unit lfu is used as a filtration device the lfu comes pre-inserted into the jetstar ion exchange maxi column or the new jetstar ion exchange midi column and the uncleared bacterial lysate is simply applied into the lfu after equilibration the lysate passes the lfu membrane and the plasmid dna is simultaneously bound to the ion exchange resin in the jetstar column the lfu is removed from the column and discarded the column is washed with wash buffer to remove residual impurities thereafter the purified dna is eluted from the column with elution buffer the eluted dna is ready to be precipitated with isopropanol lysate filtration unit lfu jetstar lfu maxi column prepare your bacterial lysate alkaline/sds lysis and potassium acetate neutralization as usual but no centrifugation and add it into the lysate filtration unit lfu pre-inserted into a jetstar midi or maxi column the flow starts automatically liquid and plasmid dna pass the lfu membrane and the dna is immediately bound to the iex resin of the jetstar column all bacterial debris and sds precipitates are collected at the lfu bottom 1 2 3 after approximately 15 min the lysate has passed the lfu and the plasmid dna is completely bound to the iex resin of the jetstar column the lfu is removed and discarded the jetstar column is washed with buffer after washing the dna is eluted from the jetstar column with elution buffer the eluted dna is ready to be precipitated with isopropanol steps 1 to 3 5 min are lysate filtration steps all other steps follow the common jetstar procedure page 10 17 www.genomed-dna.com 13

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