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the most comprehensive line for behavioral research locomotor activity exploration sensory motor coordination analgesia learning memory anxiety depression reward addiction food drink/metabolism call to receive other cell biology electrophysiology animal organ cell physiology behavioral research catalogs of interest micro/nano fluidics electroporation electrofusion molecular sample preparation

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panlab now a subsidiary of harvard apparatus manufactures and distributes high quality equipment for the biological sciences for over 30 years our team covers all stages of development ­ from design to manufacturing software to hardware technical and scientific support panlab/harvard apparatus is your reliable source for the most comprehensive solutions to your application needs our offerings include the following application areas video tracking activity exploration sensory motor analgesia memory anxiety metabolism non-invasive blood pressure and isolated physiology systems our team is ready to adapt improve or develop new or pre-existing lines to meet our customer s changing requirements modular operant box superior versatility see page 75 startle/fear combined system advanced technology for better results see page 71 smart with m ultip excepti onal vid le arena exten sion eo track ing dete softwar ction e see p age 6 species guide for behavioral systems rabbit guinea pig rat mouse insect fish to help you become more familiar with our product line panlab/harvard apparatus has added small animal icons for every product these icons will help guide you to which species can be used with each system above is a sample of the animal icons you will see throughout this catalog harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 1 acquisition behavioral research catalog

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table of contents products video tracking smart video-tracking system .6 ­ 7 smart junior video-tracking system .8 ­ 9 activity exploration ir actimeter for activity exploration .15 ­ 16 actitrack software ir actimeter .17 sensory motor rota rods for motor coordination .22 grip strength meters .23 ­ 24 rodent activity wheel .25 ­ 26 treadmills .27 ­ 28 rotameter .29 rodent shocker .30 analgesia locomotor activity and exploration selection guide10 ­ 14 sensorimotor and coordination selection guide .18 ­ 21 spatial place preference box analgesia selection guide .31 ­ 34 locomotor activity and exploration selection guide tail flick analgesia meter .35 ­ 36 hot and hot/cold plate meters .37 39 thermal place preference/gradient tests .40 ­ 41 electronic von frey .42 heat-flux infrared radiometer .43 plantar tests .44 ­ 46 rat paw pressure analgesia meter .47 plethysmometer for evaluating paw volume .48 ­ 49 dynamic weight bearing test .50 pressure application measurement pam .52 pressure application measurement pam .52 page no selection guide .5 smart videotracking system

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oxylet system for respiratory metabolism products continued memory attention page no actitrack software ir actimeterrelated tracking anxiety locomation exploration learning and memory selection guide .53 ­ 60 attention selection guide .61 ­ 62 shuttle boxes for active/passive avoidance .63 ­ 64 shutavoid software for active passive avoidance .65 ­ 66 circular pool .67 ­ 68 radial maze mazesoft-8 software .69 ­ 70 startle and fear system software .71 ­ 74 modular operant box for operant conditioning .75 ­ 76 packwin software .77 ­ 78 5/9 holes for attention performance .79 passive avoidance box .80 ­ 81 anxiety depression anxiety selection guide .82 ­ 85 depression selection guide .86 ­ 87 elevated plus maze mazesoft-4 software .88 ­ 89 open field black and white boxes .90 aron test for screen anxiolytic substances .91 black and white test for evaluating anxiety .92 vogel test for screening anxiolytic effects of drugs .93 tail suspension test for screening antidepressant activity .94 addiction reward reward addiction selection guide .95 97 place preference boxes .98 spatial place preference .99 ppcwin software .100 self-administration box .101 small animal treadmill metabolism food and drink/metabolism selection guide .102 ­ 104 oxylet system for respiratory metabolism .105 ­ 107 metabolism software .108 phecomp .109 ­ 110 harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 3

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do you have a technical question our staff of scientists have the answers you need in addition to our high quality research products panlab/harvard apparatus is proud to offer unparalleled technical sales for both pre and post-sales our technical staff scientists are happy to help answer any questions you may have or assist with system configurations contact us or visit our websites for access to research articles product specifications working procedures 4 demonstration videos instruction manuals application sheets www.harvardapparatus.com or www.panlab.com harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com

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selection guide research area video tracking activity exploration experimental test video-tracking studies locomotor activity/rearing open field test calorimetry food drink activity rearing hole-board test response to novelty voluntary exercise coordination and equilibrium test rotation after lesioning grip strength exercise training startle response tail flick test hot plate test hot/cold plate test randall selitto test inflammation incapacitance test von frey test passive avoidance test active avoidance test aron test morris water maze radial maze test t-maze test object recognition test odor recognition test fear conditioning test fear potentiated startle reflex test operant procedures 5/9 hole test prepulse inhibition of startle reflex 5/9 hole test operant procedures open field test locomotor activity/rearing elevated plus maze test black and white box test vogel test geller-seifter test forced swimming test tail suspension test place preference test self-administration social interaction respiratory metabolism food drink intake compulsive behavior hardware frame grabber board camera ir actimeter open field chamber oxylet ir actimeter open field chamber rodent activity wheel rota rod rotameter grip strength meter treadmill startle and freezing combined system tail flick meter hot plate meter hot/cold plate test meter rat paw pressure plethysmometer incapacitance test meter electronic vonfrey passive box shuttle box aron box circular pool radial maze t-maze open field chamber startle and freezing combined system startle and freezing combined system modular operant box 5/9 hole box startle and freezing combined system 5/9 hole box modular operant box open field chamber ir actimeter elevated plus maze black and white box vogel test modular operant box cylinder tail suspension test system place preference box spatial place preference self-administration box open field chamber oxylet foodrink phecomp phecomp software smart smart and smart junior sedacom or actitrack smart or smart junior metabolism sedacom or actitrack smart or smart junior multicounter or sedacom sedacom sedacom sedacom sedacom startle software sedacom sedacom sedacom sedacom sedacom sedacom sedacom programmer or shutavoid programmer or shutavoid smart or smart junior mazesoft-8 or smart smart or smart junior smart smart freezing software startle software packwin packwin startle software packwin packwin smart or smart junior actitrack smart or smart junior ppcwin or smart packwin packwin smart ppcwin ppcwin or smart packwin smart with social interaction module metabolism and meta-oxy module metabolism and meta-int module compulse compulse sensory motor analgesia pain learning memory attention anxiety depression addiction reward social behavior metabolism

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video tracking smart video-tracking system for automated recording of animal behavior key features ® flexible and precise analysis of animal behavior ® optimized tracking in low contrast conditions ® highly user-friendly ® automated detection of head center mass and base-tail with triwise technology option ® digital video file analysis capabilities ® entirely configurable data report ® day/night cycle control system ® zone-dependent camera settings ® immobility detection for forced-swimming test and freezing ® user defined criteria for zone entry with triwise technology option smart is a complete and user-friendly video-tracking system for evaluating behavior in experimental animals it allows the recording of activity trajectories events social behavior interactions and performs the calculations of a wide range of analysis parameters the system offers flexible and easy to learn interface for setting up a wide variety of behavioral tests water maze open field plus/radial arm mazes and place preference tests in addition to other user-designed applications smart works with animals located in up to 16 separate enclosures providing both quantitative and qualitative analysis of each animal s path each animal enclosure can be divided into different zone of interest using the specific tools provided by smart up to 31 different zones and one exclusion zone can be easily drawn with different name and characteristics standard target arm or hidden a special tool for water maze is included animal trajectories are acquired from real time tv images or videotaped records and stored enabling you to analyze and reanalyze experiments with different zone configurations and parameters tracking allows not only data acquisition of the spatial position of the animal but also the automatic detection of a range of specific behaviors manually scored behaviors e.g grooming can be calculated for any zone or independent variable the parameters evaluated are presented in reports entirely configurable by the user the report coverage can be the full track or it can be split into different intervals of time results can be directly and automatically exported to excel® smart can elaborate a graphic representation/image of the tracks studied this option is of great interest to illustrate data in publications and for conferences an adapted version of smart smart-dt is provided for free to check data generate statistics print out results and obtain graphics smartdt can be installed in as many computers as may be required the smart system can be expanded for using the triwise technology for the automated detection of the head center-mass and base-tail allowing then a more detailed evaluation of some specific behavioral items rearing rotations object exploration entries into zones contacts and more parameters measured ® animal trajectory distance speed permanence time in zone etc ® specific parameters for morris water maze latency to target time near walls wishaw s error permanence time in quadrants directionality etc ® social interaction contacts relative movements etc ® immobility periods ® global activity ® rearing input/output or triwise option ® events visualized by the experimenter using event recorder ® clockwise and counter clockwise rotations triwise option 6 harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com

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video tracking components included ® cables and connectors ® instruction manual smart video-tracking system for automated recording of animal behavior continued ® cd and usb protection key ® free software updates of the acquired system ® 2 year warranty on hardware specifications computer requirements 2 ghz processor or higher celeron processor not supported 2 gb of ram with pci 32-bit bus master expansion slot available and 1 free usb port via chipset not recommended 256 colors palette graphics card for 1024x768 pixels 32-bit true color rgb display windows® 98 2000 xp sp2 or higher vista 32 video camera video tape dvd player or digital video files graphic card system requirements sources options ® a wide range of cameras and accessories are available please contact our technical support staff for details ® material for camera fixation upon request ® dvd reader/writer ® pc station ® telemetric switch remote start/stop switch ® frame grabber board pc interface board ® multiplexers digital switches digital recorder are available upon request order model bh2 76-0028 smart-bs bh2 76-0501 frboard2 product smart video-tracking system v 2.5 single subject tracking needs frboard frame grabber board pc interface board options bh2 76-0265 smart-ma related hardware multiple arenas extension one animal per arena in up to 16 arenas social behavior extension up to 16 animals in a single arena triwise module for detection of head center mass and base-tail smart system extension control box for 8 inputs and 8 outputs smart system extension control box for 32 inputs and 32 outputs upgrade from previous versions to v 2.5 telemetric switch remote start/stop switch bh2 76-0266 smart-ss bh2 76-0327 smart tw bh2 76-0267 smart i/o 8c bh2 76-0268 smart i/o 32c bh2 76-0269 smart upg2.5 bh2 76-0270 smart ts ® open field box see page 90 ® radial maze see page 69 ® circular pool see pages 67 ­ 68 ® elevated plus maze see page 88 ® black white box see page 92 ® many other possibilities ® spatial place preference box see page 99 note citations we offer a full line of mazes haploinsufficient for the dual specificity tyrosine-regulated kinase-1a dyrk1a plos one 37 e2575 water maze mouse spain das sr et al 2008 relationship between mrna expression of splice forms of the _1 subunit of the n-methyl-d-aspartate receptor and spatial memory in aged mice brain research 1207:142-154 working memory task mouse usa fan lw et al 2008 alfa-phenyl-n-tert-butyl-nitrone ameliorates hippocampal injury and improves learning and memory in juvenile rats following neonatal exposure to lipopolysaccharide eur j neurosci 276 1475-1484 open-field plus-maze rat taiwan feiyong jia et al 2008 blocking histamine h1 improves learning and mnemonic dysfunction in mice with social isolation plus repeated methamphetamine injection j pharmacol sci 107 167-174 water maze mouse japan hook vyh et al 2008 inhibitors of cathepsin b improve memory and reduce beta-amyloid in transgenic alzheimer s disease mice expressing the wild-type but not the swedish mutant beta-secretase app site j biochem chem 28312 7745-53 water maze mouse usa mcclean j et al 2008 17 estradiol is neuroprotective in male and female rats in a model of early brain injury experimental neurology 2101 41-50 water maze rat usa rothstein s et al 2008 response to neonatal anesthesia effect of sex on anatomical and behavioral outcome neuroscience 1524 959-969 rat usa pastor r et al 2008 ethanol injected into the hypothalamic arcuate nucleus induces behavioral stimulation in rats an effect prevented by catalase inhibition and naltrexone behavioural pharmacology 197 698-705 locomotor activity rat spain ruiz-medina j et al 2008 intracranial self-stimulation facilitates a spatial learning and memory task in the morris water maze neuroscience 1542 424-430 water maze rat spain stone ea et al 2008 an anti-immobility effect of exogenous corticosterone in mice european journal of pharmacology 5801-2 135-142 open-field mouse usa younbyoung c et al 2008 effect of acupuncture on anxiety-like behavior during nicotine withdrawal and relevant mechanisms neuroscience letters 4302 98-102 locomotor activity rat republic of korea folven ki et al 2009 does selenium modify neurobehavioural impacts of developmental methylmercury exposure in mice environmental toxicol pharmacol 281 111-119 motor function mouse norway new zeland griesbach gs et al 2009 controlled contusion injury alters molecular systems associated with cognitive performance j neurosci res 873 795-805 water maze rat usa handattu sp et al 2009 oral apolipoprotein a-i mimetic peptide improves cognitive function and reduces amyloid burden in a mouse model of alzheimer s disease neurobioly of disease 343 525534 water maze mouse usa harada n et al 2009 functional analysis of neurosteroidal oestrogen using gene-disrupted and transgenic mice j neuroendocrinol 214 365-369 parallelism index mouse japan hazane f et al 2009 behavioral perturbations after prenatal neurogenesis disturbance in female rat neurotoxicity res 154 1476-3524 locomotor activity rat france lee yk et al 2009 protective effect of the ethanol extract of magnolia officinalis and 4-omethylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity j nat med 633 274-282 water maze mouse korea malone dt et al 2009 cannabidiol reverses the reduction in social interaction produced by low dose delta9-tetrahydrocannabinol in rats pharmacol biochem behav 932 91-96 open-field rat australia singer hs et al 2009 prenatal exposure to antibodies from mothers of children with autism produces neurobehavioral alterations a pregnant dam mouse model elevated-plus maze mouse usa arqué g et al 2008 impaired spatial learning strategies and novel object recognition in mice harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 7

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video tracking smart junior right to the point video-tracking components included key features ® instruction manual ® simplest video-tracking system available nine steps and you re done ® free software updates of the acquired system options ® ready-to-use configurations provide concrete meaningful data ® telemetric switch remote start/stop switch ® webcam or other cameras ® pc station or laptop upon request designed for water maze experiment ® latency to reach the platform and times platform is crossed ® permanence time distance traveled speed and number of entries and into zones platform pool quadrants border and total ® movement pattern and manually scored events new smart junior ma extension with the new ma multiple arenas extension to smart junior users are able to work with an amazing number of subjects over a 100 for maximum efficiency create arenas and related zones in less than 10 clicks allows independent or synchronized all arenas or only selected arenas start/stop of tracking process the only system available that features different lighting/contrast and timing control settings for each arena open field experiment ® permanence time distance traveled speed and number of entries and into zones center periphery and border ® movement pattern and manually scored events plus maze experiment smart junior ® permanence time distance traveled speed and number of entries and center open arms and closed arms ® movement pattern and manually scored events smart junior is an economical video-tracking system specially intended for laboratories with very precise interests and needing concise meaningful reports with a more competitive price the smart junior software fulfills all the basic functions of a classic video-tracking system ready-to-use configurations run panel and data reports are directly targeted to specific standard experiments an innovative scheduler tool allows managing the subjects and trials in different phases and sessions for an easy retrieval and organization of the final data succinct graphs provide a direct visualization of the results obtained in the different experimental groups smart junior takes advantage of the latest technologies in terms of image processing for providing accurate data in the context of behavioral research the software platform is expandable new readyto-use protocol configurations can be easily plugged-in for widening the scope of the system smart junior represents the finest solution for completing molecular and cellular studies by standard behavioral analysis it is also a perfect alternative for labs just beginning behavior studies t/y maze ® alternations number max first arm choice latency first choice ® permanence time distance traveled speed and number of entries and into correct/incorrect arms left/right arms or a/b/c arms ® movement pattern and manually scored events place preference ® permanence time absolute and relative distance traveled speed and number of entries and into compartment associated to drug/placebo and corridor ® movement pattern and manually scored events 8 harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com

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video tracking smart junior related hardware right to the point video-tracking continued ® circular pool see pages 67 ­ 68 ® open field box see page 90 ® y or t maze by request ® elevated plus maze see page 88 ® place preference see page 98 specifications computer requirements graphic card requirements system requirements images sources 2 ghz processor or higher celeron processor not supported 2 gb of ram 256 colors palette graphics card for 1024x768 pixels 32-bit true color rgb display windows® xp sp2 or higher vista 32 webcam digital video firewire/usb digital camera in general any device compatible with windows® image acquisition wia faq s 1 when my trial version expires what should i do to acquire a license just follow the instructions indicated by the activation assistant of the smart junior and you will get your registered copy within minutes 2 once i buy the system will i have to pay anything else such as annual license or technical/scientific support panlab/harvard apparatus includes all related expenses in its price technical and scientific support is always available for our team 3 now there are only 5 experimental modules but do you plan to add new ones if so which ones as long as there are standard experiments with highly standardized parameters to look at we will not stop widening junior s scope 4 if at a later stage i become interested in a highly sophisticated video tracking system such as your renowned smart will i have to pay for a brand new system at panlab/harvard apparatus we make this transition simple for our customers what is more we offer a highly affordable transfer fee for customers wishing to move from smart junior to smart contact our technical support staff for more information order model product bh2 76-0029 smart junior smart junior platform needs experiment modules bh2 76-0255 sjwm bh2 76-0256 sjpm bh2 76-0257 sjof bh2 76-0416 sjty bh2 76-0415 sjpp bh2 76-0508 sjma water maze experiment module plus-maze experiment module open-field experiment module t-y maze experiment module place preference experiment module multiple arenas module options bh2 76-0270 smart remote bh2 76-0260 camweb bh2 76-0261 camweb2 bh2 76-0262 convanausb telemetric switch logitech quickcam express 5000 640x480 30fps w/cable usb 1.8 m high resolution webcam creative webcam nx ultra 640 x 480 w/cable usb 1.8 m video converter analog/digital citation camarasa j et al 2008 memantine prevents the cognitive impairment induced by 3,4methylenedioxymethamphetamine in rats eur j pharmacol 5891-3 132-9 open-field water maze rats spain harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 9

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locomotor activity exploration guide locomotor activity refers to the movement from one location to another in rodents one of the most important components of exploration a prominent activity of the animal s repertoire of spontaneous activity is locomotion moreover locomotor activity and exploration are involved in many behavioral and physiological functions and are influenced by many external factors such as environmental conditions light temperature noise and novelty and internal factors such as circadian rhythm food or drink-deprivation prior handling by the researcher age gender strain and many other factors development of behavioral measurements of locomotor activity and exploration was in part relevant in various rodent models as an initial screen for pharmacological effects predictive of therapeutic drug efficacy in humans indeed locomotor and exploration are mediated by neurotransmitters affected by many types of drugs such as neuroleptic benzodiazepines opiates and psychostimulants and consequently are changed in response to the administration of these drugs moreover alterations of locomotor activity and exploration can have important consequences for paradigms that aim to study more specific processes such as learning memory reward anxiety and others thus it is imperative to verify if a difference in drug lesion strain or genetic manipulation influences general motor activity furthermore locomotor abnormalities are associated with several human diseases such as parkinson s and huntington s disease or hyperactivity syndrome and are therefore displayed by animal models 10

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locomotor activity exploration guide behavioral test behavioral test circadian locomotor activity rodents typically display circadian rhythmic variations defined as a 24-hour rhythm in behavior and physiology circadian global activity is directly assessed in the animal home cage through long periods 24 hours the activity parameters locomotion exploration stereotypies are generally evaluated using automated procedures ex photocell beams video tracking systems or weight transducers open field test for basal global activity basal locomotor activity is generally assessed in a specific activity arena can be an open field or other for short or intermittent periods 1 hour locomotion horizontal activity exploration rearing and object sniffing and stereotypies movement without displacement are then recorded manually or using various automated procedures photocell beams videotracking system or weight transducers reasons for choosing this test ® no need for habitation not stressful familiar environment for the subject ® long-term observation of activity circadian rhythm ® easy to perform tests ® no animal handling needed ® sensitive for both mice and rats reasons for choosing this test ® ® ® ® ® free exploration paradigm widely used in literature easy to use for inexperienced users can be entirely automated sensitive for both mice and rats related human disease/applications ® drug screening ® phenotyping ® attention-deficit hyperactivity disorder reasons for not choosing this test ® requires non-stressful conditions habituation and low lighting conditions related human disease/applications ® ® ® ® ® drug screening phenotyping parkinson s disease huntington s disease attention-deficit hyperactivity disorder adhd harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 11

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locomotor activity exploration guide behavioral test behavioral test activity wheel the rodent activity wheel represents a very simple and clever way to register animal physical activity in its home cage environment the use of this high throughput tool is particularly relevant for research involving circadian rhythms phenotyping and drug testing typically the time and distance run on a voluntary running wheel are monitored over several days or weeks to determine whether a particular substance or experimental manipulation has an effect on exercise behavior locomotor response to novelty locomotor response to novelty is an index of animal exploration/anxiety that has been shown to represent a predictive factor for the addictive properties of a drug in an animal model for vulnerability to drug abuse animals that exhibit greater motor activity in a novel environment high responders hr are found more sensitive to drugs of abuse and are more likely to self-administer these drugs compared to less reactive animals low responders lr in the light of clinical evidence on comorbiity between drug abuse and mood disorders this model is widely used to investigate whether individual differences in locomotor reactivity to novelty are related to anxiety and depressionlike responsiveness in rodents reasons for choosing this test ® rodent voluntary exercise registering allows animals to exercise when and at the intensity that they choose ® availability of a running wheel may reduce the effects of chronic stress on depression-like signs in mice ® less labor intensive than treadmill running as researchers need not to be present during wheel running ® relatively inexpensive setup ® ideal for high throughput experiments many animals can be trained at the same time ® sensitive for both mice and rats reasons for choosing this test ® explore novelty-seeking behaviors ® free exploration paradigm ® test maximizing avoidance/anxiety related behavior respect to approach/exploratory behavior ® only one exposure no habituation and quickly performed ® easy to run ® sensitive for mice and rats reasons for not choosing this test ® intensity and duration of the exercise cannot be controlled ® certain lines of transgenic mice may not engage in enough voluntary wheel running exercise to produce training adaptations ® not suitable for studies that require precise timing to explore acute post exercise adaptations intermittent running throughout the active cycle reasons for not choosing this test ® anxiogenic conditions novel environment with no possibility of escape related human disease/applications ® ® ® ® drug screening phenotyping addiction anxiety disorders related human disease/applications ® ® ® ® ® drug screening phenotyping neuromuscular disease parkinson s disease muscular dystrophy 12 harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com

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locomotor activity exploration guide behavioral test behavioral test emergence test the emergence test is a free exploration paradigm designed to reduce anxiety by providing a safe enclosure within the open field in order to assess approach or exploratory behavior in rodents briefly the open field contained a white plastic cylinder with an open end centrally located the subjects are placed into the cylinder and tested for 10 to 15 minutes the parameters commonly evaluated in this task are the latency of emergence from the cylinder the total time spent inside the cylinder the time spent exploring the cylinder and general locomotor activity novel object test the novel object test is a free exploration paradigm that provides animals the opportunity to explore a novel object in a familiar environmental context briefly an object is placed into the center of each open field and the behavior of the animal time spent exploring the novel object and overall locomotor activity is registered during a determined period of time reasons for choosing this test ® free exploration paradigm in a familiar environment ® test maximizing approach/exploratory behavior respect to avoidance/anxiety-related behavior ® easy to run even for inexperienced users ® sensitive for both mice and rats reasons for choosing this test ® free exploration paradigm in reduced anxiogenic environment ® easy to run ® sensitive for both mice and rats related human disease/applications ® drug screening ® phenotyping ® parkinson s disease related human disease/applications ® drug screening ® phenotyping ® parkinson s disease harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com 13

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locomotor activity exploration guide behavioral test behavioral test hole board test the hole board test is a specific test for evaluating exploration in rodents in this test the animal is placed on an arena with regularly arranged holes on the floor both frequency and duration of spontaneous elicited holepoking exploratory behavior are then measured manually or using automated procedures photocell beams video tracking system during a short period of time other associated behaviors can also be evaluated such as grooming rearing and locomotion treadmill the treadmill test in rodents is a useful tool with a great value in the study of functional capacity and is a validated standard model for investigations in the field of human metabolism a subject is forced to walk/run on a treadmill adjustable speed and inclination during specific periods of time this test allows the study of various physiological and behavioral functions such as long and short-term effort during exercise locomotion metabolic exchanges cardiac function motor coordination and fatigue reasons for choosing this test ® allows differentiation between inquisitive and inspective exploration ® very sensitive to drug effects ® can be completely automated ® easy to use even for inexperienced users ® sensitive for both mice and rats reasons for choosing this test ® adapted from a human test ® allows the researcher to precisely control the level of exertion ® easy to use even for inexperienced users ® sensitive for mice and rats reasons for not choosing this test related human disease/applications ® drug screening ® phenotyping ® needs repetitive daily exposure during few weeks ® requires constant vigilance by the researcher to make sure that the animals run for the entire exercise bout ® use of aversive stimuli to encourage running related human disease/applications ® ® ® ® ® ® improvement of sportive human performances oxidative stress diabetes parkinsons s disease ischemia ostopenia/osteoporosis 14 harvard apparatus · phone 508.893.8999 · toll free u.s 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com panlab harvard apparatus · spain +34934190709 · international +34834750697 · fax +34934750699 · www.panlab.com

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